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Nucleic Acids in the Environment

  • Book
  • © 1995

Overview

Editors:
  1. Jack T. Trevors

    1. Department of Environmental Biology, University of Guelph Lab. Microbial Techniques, Guelph, Canada

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  2. J. Dick Elsas

    1. DLO-Institute for Soil Fertility Research c/o IPO-DLO, Wageningen, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-x
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  2. Introduction to Nucleic Acids in the Environment: Methods and Applications

    • J. T. Trevors, J. D. van Elsas
    Pages 1-7

  3. Recovery of Bacterial Cells from Soil

    • L. R. Bakken, V. Lindahl
    Pages 9-27

  4. Extraction, Purification, and Analysis of DNA from Soil Bacteria

    • V. Torsvik, F. L. Daae, J. Goksøyr
    Pages 29-48

  5. Extraction and Analysis of Microbial DNA from Soil

    • A. Saano, E. Tas, S. Pippola, K. Lindström, J. D. van Elsas
    Pages 49-67

  6. Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants

    • K. Leung, J. T. Trevors, J. D. van Elsas
    Pages 69-87

  7. Extraction of DNA from the Phyllosphere

    • M. J. Bailey
    Pages 89-109

  8. Specific DNA Sequences for Detection of Soil Bacteria

    • X. Nesme, C. Picard, P. Simonet
    Pages 111-139

  9. PCR Amplification of DNA from Root Nodules

    • I. L. Pepper, S. D. Pillai
    Pages 141-151

  10. Extraction of DNA and RNA from Aquatic Environments

    • J. H. Paul, S. L. Pichard
    Pages 153-177

  11. PCR Amplification of DNA Recovered from the Aquatic Environment

    • A. K. Bej
    Pages 179-218

  12. Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity

    • P. A. Rochelle, J. A. K. Will, J. C. Fry, G. J. S. Jenkins, R. J. Parkes, C. M. Turley et al.
    Pages 219-239

  13. Application of the PCR for Detection of Antibiotic Resistance Genes in Environmental Samples

    • K. Smalla, J. D. van Elsas
    Pages 241-256

  14. Back Matter

    Pages 257-260
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Keywords

About this book

Molecular biological techniques such as DNA/RNA extraction and purification, and especially the polymerase chain reaction, PCR, are rapidly gaining interest also in related fields, such as microbiology or environmental sciences. They offer new approaches and opportunities for the determination of microbial cells, DNA and RNA from soils, roots, rhizospheres, sediments and aquatic environments.
Detailed protocols for these applications are described in this manual.

Reviews

"The protocols that are presented are well defined in a step-by step manner. This should allow their easy implementation in a laboratory. An attractive feature ... is the inclusion of a series of tables with suggested primers for analysis of specific genes." Quarterly Review of Biology

Editors and Affiliations

  • Department of Environmental Biology, University of Guelph Lab. Microbial Techniques, Guelph, Canada

    Jack T. Trevors

  • DLO-Institute for Soil Fertility Research c/o IPO-DLO, Wageningen, The Netherlands

    J. Dick Elsas

Bibliographic Information

  • Book Title: Nucleic Acids in the Environment

  • Editors: Jack T. Trevors, J. Dick Elsas

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-79050-8

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-540-58069-0Published: 28 April 1995

  • eBook ISBN: 978-3-642-79050-8Published: 07 March 2013

  • Edition Number: 1

  • Number of Pages: XII, 260

  • Number of Illustrations: 11 b/w illustrations

  • Topics: Geography, general, Geoecology/Natural Processes, Nature Conservation

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