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Enzymes, Receptors, and Carriers of Biological Membranes

A Laboratory Manual

  • Book
  • © 1984

Overview

Editors:
  1. Angelo Azzi

    1. Medizinisch-Chemisches Institut, Universität Bern, Bern, Switzerland

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  2. Urs Brodbeck

    1. Medizinisch-Chemisches Institut, Universität Bern, Bern, Switzerland

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  3. Peter Zahler

    1. Institut für Biochemie, Universität Bern, Bern, Switzerland

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Table of contents (20 chapters)

  1. Front Matter

    Pages I-XII
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  2. Analytical Separation Techniques

    1. Front Matter

      Pages 1-1
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    2. Electrophoretic Transfer of Chloroplast Membrane Proteins from SDS-Gels onto Nitrocellulose and Their Immunological Detection

      • A. Boschetti, J. Karlen, H. P. Michel
      Pages 3-12

    3. Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmorata by Charge Shift Crossed Immunoelectrophoresis

      • S. Stieger, U. Brodbeck
      Pages 13-19

    4. Separation of Hydrophobic Membrane Proteins by Phase Partition: Characterization by Two-Dimensional Gel Electrophoresis and Silver-Staining

      • K. J. Clemetson, M.-L. Zahno, B. Wyler
      Pages 20-29

    5. Quantitative Analysis of Plant Membrane Lipids by a Combined TLC-GLC Procedure

      • W. Eichenberger
      Pages 30-32

    6. Localization of Phosphatidylglycerol in the Membrane of Acholeplasma laidlawii

      • J. Op Den Kamp
      Pages 33-36

  5. Reconstitution

    1. Front Matter

      Pages 79-79
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    2. Reconstitution of Na, K-ATPase

      • B. M. Anner, M. M. Marcus, M. Moosmayer
      Pages 81-96

    3. Import of Proteins by Isolated Mitochondria

      • R. Hay, W. Oppliger
      Pages 97-105

    4. Isolation and Functional Reconstitution of Rat Liver Cytochrome Oxidase

      • P. Gazzotti
      Pages 106-111

  6. Spectral Techniques

    1. Front Matter

      Pages 113-113
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    2. Fluorescent Labeling of Band 3 Protein from Erythrocytes

      • R. P. Casey, P. S. O’Shea, A. Azzi
      Pages 115-119
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Keywords

About this book

This manual follows at a distance of 3 years the previous one entitled Mem­ brane Proteins, and, like its predecessor, it is the result of an International Advanced Course sponsored by FEBS, SKMB and SNG, which was held in Bern in September 1983. The experiments offered to the students in the course had to be largely up· dated or chosen from new areas of membrane research, because of the sub­ stantial and rapid development of the field. Using the protocols of the course, the participants (graduate students, postdoctoral fellows and also senior scientists), in most cases not at all ex­ pert in biomembrane research, were able to repeat all the experiments suc­ cessfully. Those few protocols which for some reason did not fulfill the role we expected were modified. These protocols have now been collected in this manual, which we are able to offer to a number of biology, biochemistry and biophysics laborato­ ries, hoping that the selected number of methods which have been success­ fully used during the Advanced Course may be useful to them. This manual is also intented for teachers of practical classes, who may use it as a text­ book and as source of selected references, collected not in the library, but in the laboratory, from the notebooks of the young researchers who have contributed so much to the success of the Course.

Editors and Affiliations

  • Medizinisch-Chemisches Institut, Universität Bern, Bern, Switzerland

    Angelo Azzi, Urs Brodbeck

  • Institut für Biochemie, Universität Bern, Bern, Switzerland

    Peter Zahler

Bibliographic Information

  • Book Title: Enzymes, Receptors, and Carriers of Biological Membranes

  • Book Subtitle: A Laboratory Manual

  • Editors: Angelo Azzi, Urs Brodbeck, Peter Zahler

  • DOI: https://doi.org/10.1007/978-3-642-70010-1

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1984

  • Softcover ISBN: 978-3-540-13751-1Published: 01 November 1984

  • eBook ISBN: 978-3-642-70010-1Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XII, 168

  • Number of Illustrations: 9 b/w illustrations

  • Topics: Analytical Chemistry, Biochemistry, general, Cell Biology

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