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Basic Exercises in Immunochemistry

A Laboratory Manual

  • Book
  • © 1979

Overview

Authors:
  1. Alois Nowotny

    1. University of Pennsylvania, Philadelphia, USA

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Table of contents (95 chapters)

  1. Front Matter

    Pages I-XIII
    Download chapter PDF

  2. Isolations and Preparations

    1. Fractionation of Serum Proteins by Ammonium Sulfate

      • Alois Nowotny
      Pages 1-3

    2. Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Column

      • Alois Nowotny
      Pages 3-8

    3. Isolation of IgG by DEAE Cellulose

      • Alois Nowotny
      Pages 8-12

    4. Dissociation of Macroglobulins with 2-Mercaptoethanol

      • Alois Nowotny
      Pages 12-14

    5. Enzymatic Cleavage of IgG

      • Alois Nowotny
      Pages 14-15

    6. Chromatographic Separation of the IgG Fragments

      • Alois Nowotny
      Pages 16-17

    7. Separation and Isolation of H and L Chains of IgG

      • Alois Nowotny
      Pages 17-19

    8. Affinity Chromatography and Its Application to Antibody Isolation

      • Alois Nowotny
      Pages 19-23

    9. Cellulose Acetate Paper Electrophoresis of Human Serum

      • Alois Nowotny
      Pages 23-26

    10. Disc Electrophoresis in Polyacrylamide Gel

      • Alois Nowotny
      Pages 26-35

    11. Separation of Human Hemoglobins by Electrofocusing

      • Alois Nowotny
      Pages 35-40

    12. Conjugation of Antibodies with Fluorescein and Rhodamine

      • Alois Nowotny
      Pages 41-44

    13. Enzymatic Labeling of Proteins with 125I

      • Alois Nowotny
      Pages 44-47

    14. Preparation of Peroxidase-Labeled Antibody

      • Alois Nowotny
      Pages 48-51

    15. Preparation of Soluble Dinitrophenyl Proteins

      • Alois Nowotny
      Pages 51-53

    16. Preparation of Dinitrophenyl- and Trinitrophenyl-Labeled Erythrocytes

      • Alois Nowotny
      Pages 54-57

    17. Isolation of Cell Membranes From Ascites Tumors

      • Alois Nowotny
      Pages 57-60

    18. Preparation and Use of Synthetic Liposomes

      • Alois Nowotny
      Pages 61-63

    19. Isolation of Murine Transplantation Antigen Preparation

      • Alois Nowotny
      Pages 63-66
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Keywords

Authors and Affiliations

  • University of Pennsylvania, Philadelphia, USA

    Alois Nowotny

Bibliographic Information

  • Book Title: Basic Exercises in Immunochemistry

  • Book Subtitle: A Laboratory Manual

  • Authors: Alois Nowotny

  • DOI: https://doi.org/10.1007/978-3-642-67356-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1979

  • Softcover ISBN: 978-3-540-09453-1Published: 01 September 1979

  • eBook ISBN: 978-3-642-67356-6Published: 06 December 2012

  • Edition Number: 2

  • Number of Pages: XIV, 314

  • Number of Illustrations: 28 b/w illustrations

  • Topics: Immunology, Chemistry/Food Science, general, Biomedicine general

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