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CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

  • Book
  • © 2001

Overview

Editors:
  1. Anthony B. Chen

    1. Genentech Inc., South San Francisco, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Wassim Nashabeh

    1. Genentech Inc., South San Francisco, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Timothy Wehr

    1. Bay Bioanalytical Laboratory, Richmond, USA

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    You can also search for this editor in PubMed   Google Scholar

  • aktuelle, wissenschaftlich hochwertige Informationen zu ausgewählten Themen der Kapillarelektrophorese

Part of the book series: CHROMATOGRAPHIA CE-Series (CHROM, volume 5)

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Table of contents (10 chapters)

  1. Front Matter

    Pages 1-6
    Download chapter PDF

  2. Capillary Electrophoresis in the Development of Recombinant Protein Biopharmaceuticals

    • A. B. Chen, E. Canova-Davis
    Pages 7-17

  3. Selection of Buffers in Capillary Zone Electrophoresis: Application to Peptide and Protein Analysis

    • G. M. Janini, H. J. Issaq
    Pages 18-26

  4. Reverse-Phase High Performance Liquid Chromatography and Capillary Zone Electrophoresis Separation of Protegrin Analogs

    • J. Chen, J. Fausnaugh-Pollitt
    Pages 27-33

  5. Use of Capillary Electrophoresis to Determine the Dilute Protein Concentration in Formulations Containing Interfering Excipients

    • S. S. Park, A. Cate, B. S. Chang
    Pages 34-38

  6. Effects of the Solution Environment on the Resolution of Recombinant Human Deoxyribonuclease Variants in Capillary Zone Electrophoresis

    • C. P. Quan, E. Canova-Davis, A. B. Chen
    Pages 39-44

  7. Recent Advances in Capillary Isoelectric Focusing

    • T. Wehr, R. Rodriguez-Díaz, M. Zhu
    Pages 45-58

  8. Separation of Enbrel® (rhuTNFR: Fc) Isoforms by Capillary Isoelectric Focusing

    • C. Jochheim, S. Novick, A. Balland, J. Mahan-Boyce, W.-C. Wang, A. Goetze et al.
    Pages 59-65

  9. Optimization, Validation, and Use of Capillary Gel Electrophoresis for Quality Control Testing of Synagis®, a Monoclonal Antibody

    • M. A. Schenerman, S. H. Bowen
    Pages 66-74

  10. Analysis of Protein Therapeutics by Capillary Electrophoresis

    • S. Ma, W. Nashabeh
    Pages 75-89

  11. Sensitive and High Resolution CE/MS/MS for Protein Identification in Complex Mixtures

    • W. Tong, J. R. Yates
    Pages 90-99

  12. Back Matter

    Pages 100-102
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Keywords

About this book

The continuing development of capillary electrophoresis (CE) is evident by the increasing number of journal articles, text books and symposia devoted to CE. This monograph is part of a continuing series covering individual areas of CE.

The Symposium 'CE in Biotechnology: Practical Applications for Protein Analysis' was held in San Francisco, California, USA on August 18, 1999. The symposium highlighted practical applications, including methods which had obtained regulatory approval for the routine analysis of marketed recombinant protein pharmaceuticals. This meeting demonstrated that CE has found a niche in the routine analysis of proteins and peptides in an industrial setting. The editors of this monograph, along with the series editor (Kevin Altria) were of the opinion that the proceedings of that meeting could provide the basis for theoretical and practical issues related to the analysis of therapeutic proteins and peptides by CE. This publication therefore consists of some of the practical applications that were presented by scientists from leading biotechnology companies. Several comprehensive review chapters, including 'CE in the development of recombinant protein biopharmaceuticals', 'Selection of buffers in CZE: application to peptide and protein analysis' and 'Recent advances in cIEF' are included as supplemental information.

This publication should be read by those seeking to develop and/or validate methods to be used for routine analysis of proteins and peptides for drug development and quality control.

Editors and Affiliations

  • Genentech Inc., South San Francisco, USA

    Anthony B. Chen, Wassim Nashabeh

  • Bay Bioanalytical Laboratory, Richmond, USA

    Timothy Wehr

About the editors

Anthony B. Chen, Ph.D. has worked for over fifteen years at Genentech Inc. South San Francisco, CA developing analytical assays for recombinant protein pharmaceuticals. He introduced the use of novel analytical applications, including CE, for routine protein analyses in the Quality Control department.

Wassim Nashabeh, Ph.D. has worked with CE for over ten years, including pre- and postdoctoral studies and CE for commercial applications. He is Associate Director, Quality Control, Genentech Inc. where he has validated several CE methods used in the lot release of marketed protein therapeutics.

Timothy Wehr, Ph.D. developed CE instrumentation and application kits as manager of the CE chemistry R&D group at Bio-Rad laboratories, Richmond, CA and has developed CE methods for biopharmaceuticals at Bay Bioanalytical Laboratory, Richmond CA.

Bibliographic Information

  • Book Title: CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

  • Editors: Anthony B. Chen, Wassim Nashabeh, Timothy Wehr

  • Series Title: CHROMATOGRAPHIA CE-Series

  • DOI: https://doi.org/10.1007/978-3-322-83021-0

  • Publisher: Vieweg+Teubner Verlag Wiesbaden

  • eBook Packages: Springer Book Archive

  • Copyright Information: Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig/Wiesbaden 2001

  • Softcover ISBN: 978-3-322-83023-4Published: 19 January 2012

  • eBook ISBN: 978-3-322-83021-0Published: 17 April 2013

  • Series ISSN: 1615-2255

  • Edition Number: 1

  • Number of Pages: 102

  • Number of Illustrations: 22 b/w illustrations

  • Additional Information: Ursprünglich als Heft einer Zeitschrift veröffentlich.

  • Topics: Engineering, general

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