Biotechnologies of Crop Improvement, Volume 2

1. Front Matter

   Pages i-xx

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2. Plant Genetic Transformation and Transgenic Crops: Methods and Applications

   • Satbir Singh Gosal, Shabir Hussain Wani

   Pages 1-23

3. Virus Induced Gene Silencing Approach: A Potential Functional Genomics Tool for Rapid Validation of Function of Genes Associated with Abiotic Stress Tolerance in Crop Plants

   • Ajay Kumar Singh, Mahesh Kumar, Deepika Choudhary, Jagadish Rane, Narendra Pratap Singh

   Pages 25-39

4. RNA Interference: A Promising Approach for Crop Improvement

   • B. Mamta, Manchikatla V. Rajam

   Pages 41-65

5. RNAi for Resistance Against Biotic Stresses in Crop Plants

   • Pradeep Kumar Jain, Ramcharan Bhattacharya, Deshika Kohli, Raghavendra Aminedi, Pawan Kumar Agrawal

   Pages 67-112

6. RNAi Approach: A Powerful Technique for Gene Function Studies and Enhancing Abiotic Stress Tolerance in Crop Plants

   • Ajay Kumar Singh, Mahesh Kumar, Deepika Choudhary, Lalitkumar Aher, Jagadish Rane, Narendra Pratap Singh

   Pages 113-127

7. Antifungal Plant Defensins: Insights into Modes of Action and Prospects for Engineering Disease-Resistant Plants

   • Jagdeep Kaur, Siva LS Velivelli, Dilip Shah

   Pages 129-140

8. Transgenic Plants for Improved Salinity and Drought Tolerance

   • Saikat Paul, Aryadeep Roychoudhury

   Pages 141-181

9. Engineering Disease Resistance in Rice

   • K. K. Kumar, E. Kokiladevi, L. Arul, S. Varanavasiappan, D. Sudhakar

   Pages 183-206

10. Genetic Transformation of Sugarcane and Field Performance of Transgenic Sugarcane

    • Gauri Nerkar, Avinash Thorat, Suman Sheelavantmath, Harinath Babu Kassa, Rachayya Devarumath

    Pages 207-226

11. Insect Smart Pulses for Sustainable Agriculture

    • Meenal Rathore, Alok Das, Neetu S. Kushwah, Narendra Pratap Singh

    Pages 227-247

12. Genetic Transformation of Millets: The Way Ahead

    • Sweta Dosad, H. S. Chawla

    Pages 249-286

13. Transgenic Research on Tomato: Problems, Strategies, and Achievements

    • Joydeep Banerjee, Saikat Gantait, Sutanu Sarkar, Prabir Kumar Bhattacharyya

    Pages 287-334

14. Genetic Transformation in Eucalyptus

    • Shuchishweta Vinay Kendurkar, Mamatha Rangaswamy

    Pages 335-366

15. Transgenic Manipulation of Glutamine Synthetase: A Target with Untapped Potential in Various Aspects of Crop Improvement

    • Donald James, Bhabesh Borphukan, Dhirendra Fartyal, V. M. M. Achary, M. K. Reddy

    Pages 367-416

16. Understanding the Phytohormones Biosynthetic Pathways for Developing Engineered Environmental Stress-Tolerant Crops

    • Sameh Soliman, Ali El-Keblawy, Kareem A. Mosa, Mohamed Helmy, Shabir Hussain Wani

    Pages 417-450

17. Transgenic Crops: Status, Potential, and Challenges

    • Tejinder Mall, Lei Han, Laura Tagliani, Cory Christensen

    Pages 451-485

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques.  Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived.  Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security.  The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world.  Our carefully chosen “case studies of important plant crops” intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

• crop improvement
• biotechnology
• abiotic stress
• biotic stress
• molecular breeding
• food security
• genetic engineering

• School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India

  Satbir Singh Gosal

• Mountain Research Centre for Field Crops, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

  Shabir Hussain Wani

Dr. Satbir Singh Gosal possesses B.Sc. (Med.) from P U Chandigarh, India and M.Sc. & Ph. D. (Plant breeding) from Punjab Agricultural University, Ludhiana, India. He was awarded Fellowships by The Royal Society London and The Rockefeller Foundation (USA) for his Post Doctoral Research at the University of Nottingham, England and John Innes Centre Norwich, England. Dr Gosal has served Punjab Agricultural University in various capacities such as Professor Biotechnology, Director School of Agricultural Biotechnology, Additional Director Research and Director of Research. He has also served FAO/IAEA, Vienna, Austria and took tissue culture expert mission to Iraq during 1997. Dr Gosal has rigorous training on ‘Biosafety of GM crops' from Dan Forth Centre for Plant Science Research, St. Louis; APHIS, EPA (USDA), USTDA, Washington DC, USA. He has been an Honorary Member of the Board of Assessors, Australian Research Council, Canberra, President Punjab Academy of Sciences, elected member (Fellow) of Plant Tissue Culture Association (India), Fellow of Indian Society of Genetics and Plant Breeding.  He is a recipient of Distinction Award by Society for the Promotion of Plant Science Research, Jaipur, India (2009), Fellow of Punjab Academy of Sciences, Advisory member of several universities/institutes in the area of biotechnology. He served as a member of Review Committee on Genetic Manipulation (RCGM) for 3 years at Department of Biotechnology (DBT), Government of India, New Delhi, and is a member of panel of experts in area of Biotechnology for National Fund for Strategic Research of Indian Council of Agricultural Research, New Delhi.  He has participated in more than 125 national/international conferences/meetings held in India, England, Scotland, Yugoslavia, Philippines, Indonesia, Thailand, The Netherlands, Malaysia, Singapore, Austria, Iraq, P R China, Australia, Mexico, Germany and USA. He has guided more than 75 (M.Sc. & Ph.D.) students for thesesresearch on various aspects of plant tissue culture and plant transformation. He executed more than 20 externally funded research projects funded by various national and international organizations such as Punjab State Government, ICAR, DBT, DAC NATP, FAO/IAEA, and The Rockefeller Foundation, USA. He has more than 200 research papers in refereed journals of high repute, 135 research papers in conference proceedings, several T.V./Radio talks, and 30 book chapters. He has co authored 5 Laboratory Manuals, one Text Book and 2 Edited Books.

Dr. Shabir Hussain Wani is an Assistant Professor cum Scientist, Plant Breeding and Genetics, at the Mountain Research Centre for Field Crops, Khudwani Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India since May 2013 till date. He received his B.Sc. in Agriculture from BhimRao Agricultural University Agra, India, M.Sc. and Ph.D. in Genetics and Plant Breeding from Central Agricultural University, Manipur, India. His Ph.D. research fetched the first prize in North zone at National level competition in India. After obtaining his Ph.D., he worked as Research Associate in the Biotechnology Laboratory, ICAR-Central Institute of Temperate Horticulture, Rangreth Srinagar, India for two years, up to October 2011. In November 2011 he joined the Krishi Vigyan Kendra (Farm Science Centre) as Programme Coordinator (i/c) at Senapati Manipur, India. He teaches courses related to plant breeding, seed science and technology, and stress breeding. He has published more than 100 scientific papers/chapters in peer reviewed journals, and books of international and national repute. He has served as Review Editor of Frontiers in Plant Sciences, Switzerland from 2015-2017. He is an editor of SKUAST Journal of Research, and LS: An International Journal of Life Sciences. He has also edited ten books on current topics in crop Improvement published by reputed publishers including CRC press, Taylor and Francis Group, USA and Springer. He is a Fellow of the Linnean Society of London and Society for Plant Research, India. He received various awards including Young Scientist Award (Agriculture) 2015, Young Scientist Award 2016, Young Achiever award 2016 by various prestigious scientific societies. He has also worked as visiting Scientist in department of Plant Soil and Microbial Sciences, Michigan State University, USA for the year 2016-17 under the Raman Post Doctoral Research Fellowship programme sponsored by University Grants Commission, Govt. of India, New Delhi. He is a member of the Crop Science Society of America.​ 

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