Authors:
- Open Access provided with a grant from the International Atomic Energy Agency
- Provides robust, user-friendly protocols for effective pre-field screening of mutant rice for enhanced heat stress tolerance
- Designed for use by plant breeders who need rapid screens to process large mutant populations, including segregating populations, advanced generations and germplasm collections
- The described protocols could be adapted to screen for heat stress tolerance in other crops
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Table of contents (5 chapters)
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Front Matter
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Back Matter
About this book
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.
The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
Authors and Affiliations
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Plant Breeding and Genetics Section, Joint FAO/IAEA Division, International Atomic Energy Agency, Vienna, Austria
Fatma Sarsu
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Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division, International Atomic Energy Agency, Vienna, Austria
Abdelbagi M.A. Ghanim, Brian P. Forster, Ivan Ingelbrecht
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School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
Priyanka Das, Rajeev N. Bahuguna, Ashwani Pareek
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Sokoine University of Agriculture, Morogoro, Tanzania
Paul Mbogo Kusolwa
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Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
Muhammed Ashraf
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Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
Sneh L. Singla-Pareek
About the authors
Fatma Sarsu
Plant Breeding and Genetics Section
Joint FAO/IAEA Division
Vienna, Austria
Abdelbagi M.A. Ghanim
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
Vienna, Austria
Priyanka Das
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
Rajeev N Bahuguna
School of Life SciencesJawaharlal Nehru University
New Delhi, India
Paul Mbogo Kusolwa
Sokoine University of Agriculture
Morogoro, United Republic of Tanzania
Muhammed Ashraf
Nuclear Institute for Agriculture and Biology (NIAB)
Faisalabad, Pakistan
Sneh Lata Singla-Pareek
Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology
New Delhi, India
Ashwani Pareek
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
Brian P. Forster
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
International Atomic Energy Agency
Vienna, Austria
Ivan Ingelbrecht
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
International Atomic Energy Agency
Vienna, AustriaBibliographic Information
Book Title: Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
Authors: Fatma Sarsu, Abdelbagi M.A. Ghanim, Priyanka Das, Rajeev N. Bahuguna, Paul Mbogo Kusolwa, Muhammed Ashraf, Sneh L. Singla-Pareek, Ashwani Pareek, Brian P. Forster, … Ivan Ingelbrecht
DOI: https://doi.org/10.1007/978-3-319-77338-4
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: International Atomic Energy Agency 2018
Hardcover ISBN: 978-3-319-77337-7Published: 23 August 2018
Softcover ISBN: 978-3-030-08432-5Published: 14 February 2019
eBook ISBN: 978-3-319-77338-4Published: 09 August 2018
Edition Number: 1
Number of Pages: XII, 39
Number of Illustrations: 6 b/w illustrations, 10 illustrations in colour
Topics: Plant Breeding/Biotechnology, Agriculture