Overview
- Provides easy-to-follow protocols for specialists in cancer biology and tissue engineering
- Details current achievements in design and application of probes, dye, and nanoparticles as well as sensors for live cell imaging of 3D tissue models
- Consolidates cross-disciplinary knowledge of modern microscopy with tissue engineering approaches
- Includes supplementary material: sn.pub/extras
Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1035)
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Table of contents (12 chapters)
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Front Matter
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Introduction: 3D Tissue Models, Methodology and Toolkit
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Front Matter
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Manufacturing
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Front Matter
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Application Methods and Protocols
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Front Matter
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Keywords
- 3D live cell imaging
- 3D tissue models
- Applications Multi-parametric imaging
- Autofluorescence imaging in cancer
- Ca2+ imaging neural
- Multi-parametric imaging cancer
- Multi-parametric imaging of pH
- Multi-parametric imaging viscosity cancer
- Multi-parametric quantitative imaging
- Quantitative imaging intestinal organoid
- Biological Microscopy
About this book
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Editors and Affiliations
About the editor
Ruslan I. Dmitriev graduated from Lomonosov Moscow State Academy of Fine Chemical Technology (MSc) and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (PhD, 2008), where he studied membrane ion-transporting proteins and protein interactions. He trained as postdoc at University College Cork, focusing on cell metabolism, hypoxia research and development and biological applications of cell-penetrating phosphorescent probes for molecular oxygen. Since 2014, he leads Metabolic Imaging group at University College Cork, where he designs novel biocompatible FLIM and PLIM biosensors for 3D tissue models for regenerative medicine and cancer biology.
Bibliographic Information
Book Title: Multi-Parametric Live Cell Microscopy of 3D Tissue Models
Editors: Ruslan I. Dmitriev
Series Title: Advances in Experimental Medicine and Biology
DOI: https://doi.org/10.1007/978-3-319-67358-5
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2017
Hardcover ISBN: 978-3-319-67357-8Published: 10 November 2017
Softcover ISBN: 978-3-319-88418-9Published: 23 June 2018
eBook ISBN: 978-3-319-67358-5Published: 26 October 2017
Series ISSN: 0065-2598
Series E-ISSN: 2214-8019
Edition Number: 1
Number of Pages: XII, 172
Number of Illustrations: 14 b/w illustrations, 39 illustrations in colour
Topics: Regenerative Medicine/Tissue Engineering, Biological Microscopy, Biomedical Engineering/Biotechnology, Biomedical Engineering and Bioengineering, Spectroscopy and Microscopy