Overview
- Sound explanation for microscopy basics
- Introduction to complex issues of application
- Answers many questions of everyday problems in scientific microscopy research
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Table of contents (9 chapters)
Keywords
About this book
This book offers a comprehensive introduction to confocal microscopy – with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an outline of fluorescence and its corresponding implications for microscopy. The following excursus on the confocal beam paths includes implementation of acousto-optical devices and modern sensor techniques. Complex relationships are explained in a comprehensible manner, supported by many graphical figures.
Discussing the principles of magnifying optics and the technical fundamentals and modes of operation of modern laser scanning microscopes, it is a valuable resource for student and lab technicians as well as faculty members.
Authors and Affiliations
About the author
Dr. Rolf Theodor Borlinghaus, born 1956, study of biology in Konstanz and promotion to Dr. rer. nat. at the biophysical institute. From 1990 product manager for complex fluorescence microscopy and confocal microscopy with Carl Zeiss Oberkochen (later Jena). Since 1997 with Leica Lasertechnik Heidelberg, now Leica Microsystems Mannheim. Today responsible for technology transfer and training.
Bibliographic Information
Book Title: The White Confocal
Book Subtitle: Microscopic Optical Sectioning in all Colors
Authors: Rolf Theodor Borlinghaus
DOI: https://doi.org/10.1007/978-3-319-55562-1
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing AG 2017
Hardcover ISBN: 978-3-319-55561-4Published: 01 June 2017
Softcover ISBN: 978-3-319-85695-7Published: 02 August 2018
eBook ISBN: 978-3-319-55562-1Published: 25 May 2017
Edition Number: 1
Number of Pages: XIII, 115
Number of Illustrations: 73 illustrations in colour
Topics: Biological Microscopy, Cell Biology, Spectroscopy and Microscopy, Optics, Lasers, Photonics, Optical Devices