Overview
- Broadens readers’ understanding of protein glycosylation profiling using capillary electrophoresis-mass spectrometry and PK studies of biologics analysis using CE-MS technologies
- Illustrates the complete process for improving protein sequence coverage in the peptide mapping of monoclonal antibody by combining LC-MS with CE-MS workflow
- Enriches understanding of the most recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics
- Includes supplementary material: sn.pub/extras
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Table of contents (7 chapters)
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Front Matter
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Back Matter
Keywords
- ADCs
- CE-MS
- Monoclonal antibody
- PK study of biologics analysis
- PTMs
- Protein glycosylation profiling
- cIEF-MS technology
- capillary electrophoresis-mass spectrometry
- characterization of antibody-drug conjugates
- combining LC-MS with CE-MS workflow
- mass spectrometry
- peptide mapping of monoclonal antibody
- posttranslational modifications
- protein sequence coverage
- recombinant therapeutic proteins
About this book
This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.
Editors and Affiliations
About the editors
James Qiangwei Xia, Ph.D., is the CEO and principal scientist of CMP Scientific, Corp. in Brooklyn, New York. His current research interest is mainly the electrophoretic separation and mass spectrometric detection of analytes that are relevant to life science and biopharmaceutical industry. More specifically, he is devoted to making capillary electrophoresis - mass spectrometry (CE-MS) coupling technology simpler, faster, and more robust.
Lichao Zhang, Ph.D., is the staff scientist of Stanford University, California. She graduated from the University of Virginia with a Ph.D. degree in the area of mass spectrometry-based proteomics. Her current research interest falls into the application of mass spectrometry to the identification and quantification of proteins out of complex biological samples.
Bibliographic Information
Book Title: Capillary Electrophoresis-Mass Spectrometry
Book Subtitle: Therapeutic Protein Characterization
Editors: James Q. Xia, Lichao Zhang
DOI: https://doi.org/10.1007/978-3-319-46240-0
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing Switzerland 2016
Hardcover ISBN: 978-3-319-46238-7Published: 15 November 2016
Softcover ISBN: 978-3-319-83475-7Published: 28 June 2018
eBook ISBN: 978-3-319-46240-0Published: 08 November 2016
Edition Number: 1
Number of Pages: IX, 74
Number of Illustrations: 7 b/w illustrations, 21 illustrations in colour
Topics: Protein Science, Mass Spectrometry, Spectroscopy and Microscopy