Overview
- Provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible
- Allows the novice user to truly understand the best options for specific protocol steps, reagents and materials
- Techniques presented are supported by the author’s twenty-plus years of first-hand experience working with this assay
- Includes supplementary material: sn.pub/extras
Part of the book series: Techniques in Life Science and Biomedicine for the Non-Expert (TLSBNE)
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Table of contents (10 chapters)
Keywords
About this book
This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.
The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.
Authors and Affiliations
About the author
Bibliographic Information
Book Title: Elispot for Rookies (and Experts Too)
Authors: Sylvia Janetzki
Series Title: Techniques in Life Science and Biomedicine for the Non-Expert
DOI: https://doi.org/10.1007/978-3-319-45295-1
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing Switzerland 2016
Hardcover ISBN: 978-3-319-45293-7Published: 21 November 2016
Softcover ISBN: 978-3-319-83260-9Published: 05 July 2018
eBook ISBN: 978-3-319-45295-1Published: 10 November 2016
Series ISSN: 2367-1114
Series E-ISSN: 2367-1122
Edition Number: 1
Number of Pages: VIII, 93
Number of Illustrations: 7 b/w illustrations, 32 illustrations in colour
Topics: Immunology, Cell Biology, Laboratory Medicine