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  • © 2016

Modern Proteomics – Sample Preparation, Analysis and Practical Applications

  • Provides a blueprint for scientists wishing to perform proteomics for the first time
  • Furthers the education for those who have not yet mastered the technique
  • Ties in various aspects of proteomics, including software, instruments, and emerging technologies, to give experienced researchers new insight into the potential applications of proteomics

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 919)

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Table of contents (24 chapters)

  1. Front Matter

    Pages i-ix
  2. Sample Preparation Strategies for Proteomics

    1. Front Matter

      Pages 1-1
    2. Proteomes, Their Compositions and Their Sources

      • Anna Kwasnik, Claire Tonry, Angela Mc Ardle, Aisha Qasim Butt, Rosanna Inzitari, Stephen R. Pennington
      Pages 3-21
  3. Mass Spectrometry for Proteomics Analysis

    1. Front Matter

      Pages 145-145
    2. Database Search Engines: Paradigms, Challenges and Solutions

      • Kenneth Verheggen, Lennart Martens, Frode S. Berven, Harald Barsnes, Marc Vaudel
      Pages 147-156
    3. Mass Analyzers and Mass Spectrometers

      • Anthony M. Haag
      Pages 157-169
  4. Bioinformatic Tools for Proteomics data Analysis and Interpretation

    1. Front Matter

      Pages 201-201
    2. Platforms and Pipelines for Proteomics Data Analysis and Management

      • Marius Cosmin Codrea, Sven Nahnsen
      Pages 203-215
    3. Tandem Mass Spectrum Sequencing: An Alternative to Database Search Engines in Shotgun Proteomics

      • Thilo Muth, Erdmann Rapp, Frode S. Berven, Harald Barsnes, Marc Vaudel
      Pages 217-226
    4. Visualization, Inspection and Interpretation of Shotgun Proteomics Identification Results

      • Ragnhild R. Lereim, Eystein Oveland, Frode S. Berven, Marc Vaudel, Harald Barsnes
      Pages 227-235
    5. Protein Inference

      • Zengyou He, Ting Huang, Can Zhao, Ben Teng
      Pages 237-242
    6. Modification Site Localization in Peptides

      • Robert J. Chalkley
      Pages 243-247
    7. Useful Web Resources

      • Andre Bui, Maria D. Person
      Pages 249-253
    8. Mass Spectrometry-Based Protein Quantification

      • Yun Chen, Fuqiang Wang, Feifei Xu, Ting Yang
      Pages 255-279
    9. Bioinformatics Tools for Proteomics Data Interpretation

      • Karla Grisel Calderón-González, Jesús Hernández-Monge, María Esther Herrera-Aguirre, Juan Pedro Luna-Arias
      Pages 281-341

About this book

This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of:

1. The types of samples can be analyzed by mass spectrometry for proteomics analysis.

2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis.

3. Ways toreduce the complexity of the proteome to achieve better coverage of the constituent proteins.

4. How various mass spectrometers work and different ways they can be used for proteomics analysis

5. The various platforms that are available for proteomics data analysis

6. The various applications of proteomics technologies in biological and medical sciences

This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.

Editors and Affiliations

  • UT Southwestern Medical Center, Dallas, USA

    Hamid Mirzaei

  • Biotech Division, Neurophagy Therapeutics, INC, Odessa, USA

    Martin Carrasco

Bibliographic Information

  • Book Title: Modern Proteomics – Sample Preparation, Analysis and Practical Applications

  • Editors: Hamid Mirzaei, Martin Carrasco

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-3-319-41448-5

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2016

  • Hardcover ISBN: 978-3-319-41446-1Published: 28 December 2016

  • Softcover ISBN: 978-3-319-82357-7Published: 04 May 2018

  • eBook ISBN: 978-3-319-41448-5Published: 14 December 2016

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: IX, 533

  • Number of Illustrations: 50 b/w illustrations, 130 illustrations in colour

  • Topics: Proteomics, Protein Science, Bioinformatics

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access