Overview
- Gives novel approaches to structure determination of membrane proteins
- Provides good coverage on how membrane proteins interact with their surrounding environment
- Is a useful guide not only for students, but also for experienced scientists who need to deal with very small and problematic protein crystals
Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 922)
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Table of contents (14 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Â Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins.
This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes:- Expression screening using fluorescent proteins
- The use of detergents in membrane protein research
- The use of NMR
- Synchrotron developments in membrane protein structural biology
- Visualisation and X-ray data collection of microcrystals
- X-ray diffraction data analysis from multiple crystals
- Serial millisecond crystallography
- Serial femtosecond crystallography
- Membrane protein structures in drug discovery
The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work.
Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.
Editors and Affiliations
Bibliographic Information
Book Title: The Next Generation in Membrane Protein Structure Determination
Editors: Isabel Moraes
Series Title: Advances in Experimental Medicine and Biology
DOI: https://doi.org/10.1007/978-3-319-35072-1
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing Switzerland 2016
Hardcover ISBN: 978-3-319-35070-7Published: 05 September 2016
Softcover ISBN: 978-3-319-81715-6Published: 04 May 2018
eBook ISBN: 978-3-319-35072-1Published: 23 August 2016
Series ISSN: 0065-2598
Series E-ISSN: 2214-8019
Edition Number: 1
Number of Pages: X, 184
Number of Illustrations: 73 b/w illustrations
Topics: Protein Science, Protein Structure