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  • © 2016

Immunohistochemistry

Essential Elements and Beyond

  • Identifies the proper use of each component of immunohistochemical experiments
  • Concise and intuitive guide to using immunohistochemical techniques
  • Technique most useful for the following types of experiments: producing transgenic animals, studying the effects of drug treatments, electrophysiological studies, isolating and differentiating stem cells, super-resolution microscopy
  • Includes supplementary material: sn.pub/extras

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Table of contents (9 chapters)

  1. Front Matter

    Pages i-xiv
  2. Short Historic Overview

    • Alexander E. Kalyuzhny
    Pages 1-2
  3. Primary Antibodies

    • Alexander E. Kalyuzhny
    Pages 3-9
  4. Choosing Commercial Suppliers of Primary Antibodies

    • Alexander E. Kalyuzhny
    Pages 11-14
  5. Detection and Visualization of Antigens

    • Alexander E. Kalyuzhny
    Pages 15-28
  6. Ancillary Reagents for IHC

    • Alexander E. Kalyuzhny
    Pages 29-36
  7. Preparing Tissues for IHC Staining

    • Alexander E. Kalyuzhny
    Pages 37-47
  8. Staining Protocols

    • Alexander E. Kalyuzhny
    Pages 49-61
  9. Concluding Remarks

    • Alexander E. Kalyuzhny
    Pages 75-75
  10. Back Matter

    Pages 77-85

About this book

Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments:

 Â·         producing transgenic animals

 Â·         studying the effects of drug treatments

 Â·         electrophysiological studies

 Â·         animal surgery

 Â·         isolating and differentiating stemcells

 Â·         super-resolution microscopy

Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.

Authors and Affiliations

  • University of Minnesota, MINNEAPOLIS, USA

    Alexander E. Kalyuzhny

About the author

Alexander E. Kalyuzhny graduated in 1981 from the Biological Department of the Saratov State University in Russia. After graduation, he performed research on the effects of narcotic drugs on the morphogenesis of early embryos, which he defended as his Ph.D. thesis in 1987. In 1992, Dr. Kalyuzhny was invited to join the Department of Pharmacology at the University of Minnesota (USA), where he is currently affiliated with the Department of Neuroscience. He is the author of 31 papers in peer-reviewed journals and 16 book chapters, and has served as the editor of 3 books (Humana Press/Springer). He is also the editor-in-chief of the open-access journal Cells. 

Bibliographic Information

Buy it now

Buying options

eBook USD 69.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 89.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access