Quantifying Interactions of Biomolecules with Inorganic Surfaces

1. Front Matter

   Pages i-xviii

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2. Introduction

   • Abhijeet Patra

   Pages 1-6

3. Methods

   • Abhijeet Patra

   Pages 7-25

4. Quantifying Interactions Between Water and Surfaces

   • Abhijeet Patra

   Pages 27-40

5. Quantifying Interactions Between Lipids and Surfaces

   • Abhijeet Patra

   Pages 41-67

6. Quantifying Interactions Between Serum Proteins and Gold Nanoparticles

   • Abhijeet Patra

   Pages 69-90

7. Conclusion

   • Abhijeet Patra

   Pages 91-93

8. Back Matter

   Pages 95-102

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This thesis demonstrates the adaptation of existing techniques and principles towards enabling clean and precise measurements of biomolecules interacting with inorganic surfaces. In particular, it includes real-time measurement of serum proteins interacting with engineered nanomaterial. Making meaningful and unambiguous measurements has been an evolving problem in the field of biology and its various allied domains, primarily due to the complex nature of experiments and the large number of possible interferants. The subsequent quantification of interactions between biomolecules and inorganic surfaces solves pressing problems in the rapidly developing fields of lipidomics and nanomedicine.

• Nanoscale Wettability
• Lipid Fraction Enrichment
• Surface Plasmon Resonance
• Protein Corona
• Nanoparticle-Based Drug Screening
• Serum Protein Interactions

• NUS Graduate School for Integrative Sciences and Engineering, NUS Nanoscience and Nanotechnology Institute, Singapore, Singapore

  Abhijeet Patra

Abhijeet Patra obtained his bachelors degree in Electrical and Electronics Engineering from the National Institute of Technology, Tiruchchirappalli, one of the premier institutes in India in 2011. Upon receiving the prestigious NGS scholarship to pursue doctoral studies at National University of Singapore, he joined the research group of Prof T Venkatesan, pioneer of pulsed laser deposition and, after an exciting 4 years, graduated with a PhD in 2015. His research interests are in understanding how inorganic assemblies interact with the biological world, with a sharp focus on translating the science into biomedical applications. 

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