Microfluidic Methods for Molecular Biology

1. Front Matter

   Pages i-xii

   PDF

2. Microfluidic Platforms for Quantitative Biology Studies in Model Organisms

   • Daniel A. Porto, Tel M. Rouse, Adriana San-Miguel, Hang Lu

   Pages 1-18

3. Microfluidic Methods in Single Cell Biology

   • Arnab Mukherjee, Charles M. Schroeder

   Pages 19-54

4. Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics

   • Aashish Priye, Victor M. Ugaz

   Pages 55-69

5. Forensic Typing of Single Cells Using Droplet Microfluidics

   • Tao Geng, Richard A. Mathies

   Pages 71-94

6. Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA Targets in Single Cells

   • Robert J. Meagher, Meiye Wu

   Pages 95-112

7. Microfluidic Multistage Integration for Analysis of Circulating Exosomes

   • Mei He, Andrew Godwin, Yong Zeng

   Pages 113-139

8. Microfluidic Single-Cell Functional Proteomics

   • Shay Mailloux, Lisa Ramirez, Jun Wang

   Pages 141-159

9. Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

   • Wanqing Yue, Mengsu Yang

   Pages 161-187

10. Single-Cell Phenotypic Screening in Inverse Metabolic Engineering

    • A. E. Vasdekis, G. Stephanopoulos

    Pages 189-204

11. Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers

    • Chen Zhao, Martin M. Thuo, Xinyu Liu

    Pages 205-218

12. Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression

    • Pooja Sabhachandani, Saheli Sarkar, Tania Konry

    Pages 219-233

13. Wire-guided Droplet Manipulation for Molecular Biology

    • Dustin K. Harshman, Jeong-Yeol Yoon

    Pages 235-252

14. Mechanical and Electrical Principles for Separation of Rare Cells

    • Elisa M. Wasson, Temple A. Douglas, Rafael V. Davalos

    Pages 253-294

15. Microfluidics for High-Throughput Cellular Isolation and Analysis in Biomedicine

    • Caroline N. Jones, Joseph M. Martel-Foley

    Pages 295-322

16. Microfluidics for Cell Culture

    • Deepika Devadas, Edmond W. K. Young

    Pages 323-347

17. Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations

    • Yan Zhu, Chang Lu

    Pages 349-363

18. Back Matter

    Pages 365-376

    PDF

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry.

This book also:

• Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
  
• Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
  
• Broadens readers’ understanding of microfluidics convection-based PCRtechnology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
  

• cellular heterogeneity in tissues
• genetic analysis of single cells
• microfluidic PCR
• microfluidic methods for molecular biology
• microfluidic stretching of chromatins
• molecular separations using micro and nanofluidics
• biochemical engineering

• Virginia Tech, Blacksburg, USA

  Chang Lu

• Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, USA

  Scott S. Verbridge

Chang Lu is a Professor of Chemical Engineering at Virginia Tech. His research focuses on developing microfluidic biotechnologies for cellular and molecular analysis with recent emphasis on low-input epigenomic studies. Scott Verbridge is an Assistant Professor in the Virginia Tech Department of Biomedical Engineering and Mechanics, and the Virginia Tech - Wake Forest School of Biomedical Engineering and Sciences. His research is focused on developing engineered tumor models to better understand cell-microenvironment interactions, and their impact on tumor progression and therapy response.  

Policies and ethics