Overview
- Provides in-depth coverage of expression systems tailored for the production of multisubunit complexes
- Gives extensive practical advice on how to design and conduct protein complex production experiments
- Offers insightful discussions on characterization of purified protein complexes
Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 896)
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Table of contents (22 chapters)
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Front Matter
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Prokaryotic Expression Hosts
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Front Matter
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Higher Eukaryotic Expression Hosts
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Front Matter
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Keywords
About this book
This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.
Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.
This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
Editors and Affiliations
About the editor
Dr. M. Cristina Vega is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance.
Bibliographic Information
Book Title: Advanced Technologies for Protein Complex Production and Characterization
Editors: M. Cristina Vega
Series Title: Advances in Experimental Medicine and Biology
DOI: https://doi.org/10.1007/978-3-319-27216-0
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2016
Hardcover ISBN: 978-3-319-27214-6Published: 20 May 2016
Softcover ISBN: 978-3-319-80094-3Published: 26 May 2018
eBook ISBN: 978-3-319-27216-0Published: 10 May 2016
Series ISSN: 0065-2598
Series E-ISSN: 2214-8019
Edition Number: 1
Number of Pages: XIII, 382
Number of Illustrations: 16 b/w illustrations, 71 illustrations in colour
Topics: Protein Science, Protein Structure, Systems Biology