Tumor Microenvironment

1. Front Matter

   Pages i-viii

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2. Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

   • Anika Nagelkerke, Paul N. Span

   Pages 1-10

3. Measuring DNA Replication in Hypoxic Conditions

   • Iosifina P. Foskolou, Deborah Biasoli, Monica M. Olcina, Ester M. Hammond

   Pages 11-25

4. Isolation of Proteins on Nascent DNA in Hypoxia and Reoxygenation Conditions

   • Monica M. Olcina, Amato J. Giaccia, Ester M. Hammond

   Pages 27-40

5. In Vivo Interrogation of the Hypoxic Transcriptome of Solid Tumors: Optimizing Hypoxic Probe Labeling with Laser Capture Microdissection for Isolation of High-Quality RNA for Deep Sequencing Analysis

   • Lauren K. Brady, Vladimir Popov, Constantinos Koumenis

   Pages 41-58

6. Gas Chromatography Coupled to Mass Spectrometry (GC–MS) to Study Metabolism in Cultured Cells

   • Fiona Grimm, Louise Fets, Dimitrios Anastasiou

   Pages 59-88

7. Probing Cancer Cell Metabolism Using NMR Spectroscopy

   • Kate E. R. Hollinshead, Debbie S. Williams, Daniel A. Tennant, Christian Ludwig

   Pages 89-111

8. Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenase Activity in Cultured Cells

   • Ramon C. Sun, Albert Koong, Amato Giaccia, Nicholas C. Denko

   Pages 113-120

9. Measuring Autophagy in the Context of Cancer

   • Michael J. Morgan, Andrew Thorburn

   Pages 121-143

10. Methods for Studying Autophagy Within the Tumor Microenvironment

    • Sengottuvelan Murugan, Ravi K. Amaravadi

    Pages 145-166

11. Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia

    • Russell Leek, David Robert Grimes, Adrian L. Harris, Alan McIntyre

    Pages 167-196

12. Isolation of Glioma-Initiating Cells for Biological Study

    • Jing Hu, Geoffrey Markowitz, Xiao-Fan Wang

    Pages 197-209

13. Isolation of Mouse and Human Tumor-Associated Macrophages

    • Luca Cassetta, Roy Noy, Agnieszka Swierczak, Gaël Sugano, Harriet Smith, Lisa Wiechmann et al.

    Pages 211-229

14. Mouse Models of Brain Metastasis for Unravelling Tumour Progression

    • Manuel Sarmiento Soto, Nicola R. Sibson

    Pages 231-244

15. Quantification of Lung Metastases from In Vivo Mouse Models

    • Joan Chang, Janine T. Erler

    Pages 245-251

16. A Multimodal Data Analysis Approach for Targeted Drug Discovery Involving Topological Data Analysis (TDA)

    • Muthuraman Alagappan, Dadi Jiang, Nicholas Denko, Albert C. Koong

    Pages 253-268

17. Efficient Protocol for the Identification of Hypoxic Cell Radiosensitisers

    • Cho Rong Hong, Jingli Wang, Kevin O. Hicks, Michael P. Hay

    Pages 269-290

18. Back Matter

    Pages 291-297

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This volume covers the topics presented at the 3rd International Conference on Tumor Microenvironment and Cellular Stress by an international community of researchers. The conference brings together scientists to discuss different cellular and animal models of tumor microenvironment study and identify common pathways that are candidates for therapeutic intervention; stimulate collaboration between groups that are more focused on elucidation of biochemical aspects of stress biology (e.g., HIF regulation) and groups that study the pathophysiological aspects of stress pathways or engaged in drug discovery; and critically evaluate novel targets for imaging or therapeutic intervention that would be of use to the tumor microenvironment community and pharmaceutical industry.

• Tumorigenesis
• angiogenesis
• cancer stem cells
• hypoxia
• metastasis

• Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA

  Constantinos Koumenis

• Cell & Developmental Biology, Oregon Health & Science University, Portland, USA

  Lisa M. Coussens

• Department of Radiation Oncology, Stanford University School of Medic, Stanford, USA

  Amato Giaccia

• Department of Oncology, University of Oxford, Oxford, United Kingdom

  Ester Hammond

Policies and ethics