Overview
- Provides a foundational knowledge in the understanding of prokaryotic systems biology
- Written by leading experts in the field
- Enables the microbiology community to create substantive resources to address many pending unanswered questions
Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 883)
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Table of contents (16 chapters)
Keywords
About this book
This book focuses on innovative experimental and computational approaches for charting interaction networks in bacterial species. The first part of the volume consists of nine chapters, focusing on biochemical and genetics and genomics approaches including yeast two hybrid, metagenomics, affinity purification in combination with mass spectrometry, chromatin-immunoprecipitation coupled with sequencing, large-scale synthetic genetic screens, and quantitative-based mass spectrometry strategies for mapping the bacterial physical, functional, substrate, and regulatory interaction networks needed for interpreting biological networks, inferring gene function, enzyme discovery, and identifying new drug targets.
The second part comprises five chapters, covering the network of participants for protein folding and complex enzyme maturation. It also covers the structural approaches required to understand bacterial intramembrane proteolysis and the structure and function of bacterial proteins involved in surface polysaccharides, outer membrane, and envelope assembly.
This volume concludes with a focus on computational and comparative genomics approaches, especially network-based methods for predicting physical or functional interactions, and integrative analytical approaches for generating more reliable information on bacterial gene function. This book provides foundational knowledge in the understanding of prokaryotic systems biology by illuminating how bacterial genes f
unction within the framework of global cellular processes. The book will enable the microbiology community to create substantive resources for addressing many pending unanswered questions, and facilitate the development of new technologies that can be applied to other bacterial species lacking experimental data. ​ ​Editors and Affiliations
About the editors
Nevan Krogan, PhD, is a professor at the UCSF School of Medicine in the Cellular Molecular Pharmacology department. Dr. Krogan’s lab focuses on applying global proteomic and genomic approaches to formulate hypotheses about various biological processes, including transcriptional regulation, DNA repair/replication and RNA processing. His lab at UCSF is now developing and applying methodologies to create genetic and physical interactions between pathogenic organisms, including HIV, Mtb, and Dengue, and their hosts, which is providing insight into the human pathways and complexes that are being hijacked during the course of infection.
Mohan Babu, PhD, is an Assistant Professor in the Department of Biochemistry at the University of Regina. Dr. Babu currently holds a CIHR New Investigator award and is a Maud-Menten Finalist of the CIHR Institute of Genetics. He is a member of the Canadian Society of Microbiology, Human Proteome Organization, and Canadian National Proteomics Network. Dr. Babu has developed and applied advanced proteomic, functional genomic, and bioinformatic methods to characterize genome-wide protein and epistatic interaction networks in the budding yeast and gram-negative bacterium, and mammalian cell systems. His group also developed advanced proteomics using affinity purification coupled with mass spectrometry technology to analyze the molecular composition of protein complexes and interactionsÂ
related to major diseases in humans.ÂBibliographic Information
Book Title: Prokaryotic Systems Biology
Editors: Nevan J. Krogan, PhD, Mohan Babu, PhD
Series Title: Advances in Experimental Medicine and Biology
DOI: https://doi.org/10.1007/978-3-319-23603-2
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2015
Hardcover ISBN: 978-3-319-23602-5Published: 08 December 2015
Softcover ISBN: 978-3-319-79508-9Published: 21 March 2019
eBook ISBN: 978-3-319-23603-2Published: 30 November 2015
Series ISSN: 0065-2598
Series E-ISSN: 2214-8019
Edition Number: 1
Number of Pages: IX, 319
Number of Illustrations: 3 b/w illustrations, 55 illustrations in colour
Topics: Systems Biology, Proteomics, Bacteriology