Overview
- Nominated as outstanding PhD thesis by Max-Delbrück Center for Molecular Medicine, Germany
- Describes the development of a novel technology for transcriptome-wide analysis of protein-RNA interactions
- Paves the way to future genome-wide studies of post-transcriptional gene regulatory networks
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (5 chapters)
Keywords
About this book
The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
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Authors and Affiliations
Bibliographic Information
Book Title: High-Resolution Profiling of Protein-RNA Interactions
Authors: Mathias Munschauer
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-319-16253-9
Publisher: Springer Cham
eBook Packages: Engineering, Engineering (R0)
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2015
Hardcover ISBN: 978-3-319-16252-2Published: 27 March 2015
Softcover ISBN: 978-3-319-36848-1Published: 06 October 2016
eBook ISBN: 978-3-319-16253-9Published: 14 March 2015
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XXIII, 121
Number of Illustrations: 5 b/w illustrations, 20 illustrations in colour
Topics: Biomedical Engineering and Bioengineering, Systems Biology, Computational Biology/Bioinformatics