Skip to main content
SpringerLink
Log in

Advanced Time-Correlated Single Photon Counting Applications

  • Book
  • © 2015

Overview

Editors:
  1. Wolfgang Becker

    1. Becker & Hickl GmbH, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Edited by the originator of the technique
  • Written by expert users of the technique
  • Provides clear connection of instrumental principles, physical effects used, and applications in life sciences
  • Stresses applications in medicine and biology
  • Helps users from non-physics and non-engineering areas to understand the technique and optimize their experiment
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Series in Chemical Physics (CHEMICAL, volume 111)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 chapters)

  1. Front Matter

    Pages i-xxiii
    Download chapter PDF

  2. Introduction to Multi-dimensional TCSPC

    • Wolfgang Becker
    Pages 1-63

  3. TCSPC FLIM with Different Optical Scanning Techniques

    • Wolfgang Becker, Vladislav Shcheslavskiy, Hauke Studier
    Pages 65-117

  4. Fluorescence Lifetime Imaging (FLIM): Basic Concepts and Recent Applications

    • Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregido, Alix le Marois et al.
    Pages 119-188

  5. Determination of Intracellular Chloride Concentrations by Fluorescence Lifetime Imaging

    • Thomas Gensch, Verena Untiet, Arne Franzen, Peter Kovermann, Christoph Fahlke
    Pages 189-211

  6. Calcium Imaging Using Transient Fluorescence-Lifetime Imaging by Line-Scanning TCSPC

    • Samuel Frere, Inna Slutsky
    Pages 213-224

  7. Imaging Cell and Tissue O2 by TCSPC-PLIM

    • James Jenkins, Ruslan I. Dmitriev, Dmitri B. Papkovsky
    Pages 225-247

  8. FRET Microscopy: Basics, Issues and Advantages of FLIM-FRET Imaging

    • Ammasi Periasamy, Nirmal Mazumder, Yuansheng Sun, Kathryn G. Christopher, Richard N. Day
    Pages 249-276

  9. Monitoring HIV-1 Protein Oligomerization by FLIM FRET Microscopy

    • Ludovic Richert, Pascal Didier, Hugues de Rocquigny, Yves Mély
    Pages 277-307

  10. Unraveling the Rotary Motors in FoF1-ATP Synthase by Time-Resolved Single-Molecule FRET

    • Michael Börsch
    Pages 309-338

  11. Partitioning and Diffusion of Fluorescently Labelled FTY720 in Resting Epithelial Cells

    • Dhanushka Wickramasinghe, Randi Timerman, Jillian Bartusek, Ahmed A. Heikal
    Pages 339-355

  12. Probing Microsecond Reactions with Microfluidic Mixers and TCSPC

    • Sagar V. Kathuria, Osman Bilsel
    Pages 357-384

  13. An Introduction to Interpreting Time Resolved Fluorescence Anisotropy Curves

    • Steven S. Vogel, Tuan A. Nguyen, Paul S. Blank, B. Wieb van der Meer
    Pages 385-406

  14. Time-Resolved Spectroscopy of NAD(P)H in Live Cardiac Myocytes

    • Alzbeta Marcek Chorvatova
    Pages 407-434

  15. Fluorescence Lifetime Measurements of NAD(P)H in Live Cells and Tissue

    • Alex J. Walsh, Amy T. Shah, Joe T. Sharick, Melissa C. Skala
    Pages 435-456

  16. Fluorescence Lifetime Imaging of the Skin

    • Washington Y. Sanchez, Michael Pastore, Isha N. Haridass, Karsten König, Wolfgang Becker, Michael S. Roberts
    Pages 457-508

  17. Fluorescence Lifetime Imaging in Ophthalmology

    • Dietrich Schweitzer, Martin Hammer
    Pages 509-540

  18. Dynamic Mapping of the Human Brain by Time-Resolved NIRS Techniques

    • Adam Liebert, Michal Kacprzak, Daniel Milej, Wolfgang Becker, Anna Gerega, Piotr Sawosz et al.
    Pages 541-559

  19. Time-Domain Diffuse Optical Imaging of Tissue by Non-contact Scanning

    • Heidrun Wabnitz, Mikhail Mazurenka, Laura Di Sieno, Gianluca Boso, Wolfgang Becker, Katja Fuchs et al.
    Pages 561-585

  20. Breast Monitoring by Time-Resolved Diffuse Optical Imaging

    • Giovanna Quarto, Alessandro Torricelli, Lorenzo Spinelli, Antonio Pifferi, Rinaldo Cubeddu, Paola Taroni
    Pages 587-611
Back to top

Keywords

About this book

This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research.

The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Editors and Affiliations

  • Becker & Hickl GmbH, Berlin, Germany

    Wolfgang Becker

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation