Genetic Transformation Systems in Fungi, Volume 2

1. Front Matter

   Pages i-xiv

   PDF

2. Endogenous DNA: Cell Fusion

   1. Front Matter

      Pages 1-1

      PDF

   2. Anastomosis and Heterokaryon Formation

      • Martin Weichert, André Fleißner

      Pages 3-21

   3. Induction of the Sexual Cycle in Filamentous Ascomycetes

      • Jos Houbraken, Paul S. Dyer

      Pages 23-46

   4. What Have We Learned by Doing Transformations in Neurospora tetrasperma?

      • Durgadas P. Kasbekar

      Pages 47-52

3. Endogenous DNA: Repetitive Elements

   1. Front Matter

      Pages 53-53

      PDF

   2. Repeat-Induced Point Mutation: A Fungal-Specific, Endogenous Mutagenesis Process

      • James K. Hane, Angela H. Williams, Adam P. Taranto, Peter S. Solomon, Richard P. Oliver

      Pages 55-68

   3. Calculating RIP Mutation in Fungal Genomes Using RIPCAL

      • James K. Hane

      Pages 69-78

   4. Fungal Transposable Elements

      • Linda Paun, Frank Kempken

      Pages 79-96

   5. In Vivo Targeted Mutagenesis in Yeast Using TaGTEAM

      • Shawn Finney-Manchester, Narendra Maheshri

      Pages 97-104

4. Endogenous DNA: Gene Expression Control

   1. Front Matter

      Pages 105-105

      PDF

   2. RNA Silencing in Filamentous Fungi: From Basics to Applications

      • Nguyen Bao Quoc, Hitoshi Nakayashiki

      Pages 107-124

   3. RNAi-Mediated Gene Silencing in the Beta-Lactam Producer Fungi Penicillium chrysogenum and Acremonium chrysogenum

      • Carlos García-Estrada, Ricardo V. Ullán

      Pages 125-130

   4. Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus

      • Michaela Dümig, Sven Krappmann

      Pages 131-138

5. Tools and Applications: Selection Markers and Vectors

   1. Front Matter

      Pages 139-139

      PDF

   2. Expanding the Repertoire of Selectable Markers for Aspergillus Transformation

      • Khyati Dave, V. Lakshmi Prabha, Manmeet Ahuja, Kashyap Dave, S. Tejaswini, Narayan S. Punekar

      Pages 141-153

   3. Arginase (agaA) as a Fungal Transformation Marker

      • Kashyap Dave, Manmeet Ahuja, T. N. Jayashri, Rekha Bisht Sirola, Khyati Dave, Narayan S. Punekar

      Pages 155-160

   4. Transformation of Ascomycetous Fungi Using Autonomously Replicating Vectors

      • Satoko Kanematsu, Takeo Shimizu

      Pages 161-167

   5. A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma

      • Thiago M. Mello-de-Sousa, Robert L. Mach, Astrid R. Mach-Aigner

      Pages 169-174

   6. Split-Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi

      • Kuang-Ren Chung, Miin-Huey Lee

      Pages 175-180

Fungi are an economic very important class of microbes. Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). Moreover, fungal pathogens can cause great damage in agricultural production (Phytophthora and Botrytis) and during mammalian infections (Penicillium marneffei and Candida). Transformation of DNA is used to understand the genetic basis behind these traits. Several different techniques have been developed over the years and readily shown to be decisive methods to improve fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples to be used in laboratories worldwide.

• fungi
• protocols
• reviews
• tools
• transformation
• Systems Biology

• Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

  Marco A. van den Berg

• Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

  Karunakaran Maruthachalam

Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA

Policies and ethics