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  • © 2015

Genetic Transformation Systems in Fungi, Volume 1

  • The most comprehensive book on fungal transformation methods
  • The best protocols to be applied in your lab
  • Includes the latest tools and techniques

Part of the book series: Fungal Biology (FUNGBIO)

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Table of contents (27 chapters)

  1. Front Matter

    Pages i-xv
  2. Introduction

    1. Front Matter

      Pages 1-1
  3. Transformation Methods: Protoplast Transformation

    1. Front Matter

      Pages 19-19
    2. Protoplast Transformation for Genome Manipulation in Fungi

      • Aroa Rodriguez-Iglesias, Monika Schmoll
      Pages 21-40
    3. Trichoderma Transformation Methods

      • Mónica G. Malmierca, Rosa E. Cardoza, Santiago Gutiérrez
      Pages 41-48
    4. Transformation of Mucor circinelloides f. lusitanicus Protoplasts

      • Victoriano Garre, José Luis Barredo, Enrique A. Iturriaga
      Pages 49-59
    5. Transformation of Saccharomyces cerevisiae: Spheroplast Method

      • Shigeyuki Kawai, Kousaku Murata
      Pages 61-63
  4. Transformation Methods: Electroporation

    1. Front Matter

      Pages 65-65
    2. Chemical Transformation of Candida albicans

      • Sophie Bachellier-Bassi, Christophe d’Enfert
      Pages 81-85
    3. Electroporation of Pichia pastoris

      • Knut Madden, Ilya Tolstorukov, James Cregg
      Pages 87-91
  5. Transformation Methods: Particle Bombardment

    1. Front Matter

      Pages 99-99
    2. Biolistic Transformation for Delivering DNA into the Mitochondria

      • Arianna Montanari, Monique Bolotin-Fukuhara, Mario Fazzi D’Orsi, Cristina De Luca, Michele M. Bianchi, Silvia Francisci
      Pages 101-117
    3. Biolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants

      • Jingwen Zhou, Liming Liu, Guocheng Du, Jian Chen
      Pages 119-127
    4. Use of the Biolistic Particle Delivery System to Transform Fungal Genomes

      • V. S. Junior Te’o, K. M. Helena Nevalainen
      Pages 129-133
    5. Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment

      • Eiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu, Jun Ogawa
      Pages 135-140
  6. Transformation Methods: Agrobacterium-Mediated Transformation

    1. Front Matter

      Pages 141-141
    2. Agrobacterium tumefaciens-Mediated Transformation

      • Rasmus John Normand Frandsen
      Pages 143-162

About this book

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ​

Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

Editors and Affiliations

  • Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  • Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

About the editors

Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA

Bibliographic Information

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access