Microsystem Technology

1. Front Matter

   Pages I-XXIV

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2. Molecular building principles in nature

   • J. Michael Koehler, Hans Peter Saluz

   Pages 1-15

3. Robotic equipment and microsystem technology in biological research

   • Holger Eickhoff, Igor Ivanov, Markus Kietzmann, Elmar Maier, Markus Kalkum, David Bancroft et al.

   Pages 17-30

4. Components and systems for microliquid handling

   • Steffen Howitz

   Pages 31-73

5. Nanotiterplates for screening and synthesis

   • Günther Mayer, Klaus Wohlfart, Andreas Schober, J. Michael Köhler

   Pages 75-128

6. Chip technology for micro-separation

   • Andrew J. de Mello, Andreas Manz

   Pages 129-177

7. Rapid multisample PCR in miniaturized ultrathin-walled microwell plates

   • Alexander N. Tretyakov, Rimma A. Pantina, Oleg K. Kaboev, Hans Peter Saluz

   Pages 179-187

8. Generation of large libraries

   • Carola Burgtorf, Hans Lehrach

   Pages 189-223

9. Generation and screening of solution-phase synthetic peptide combinatorial libraries

   • Andrew Wallace, Kenneth S. Koblan, Riccardo Cortese, Jackson B. Gibbs, Antonello Pessi

   Pages 225-240

10. Using oligonucleotide probe arrays to access genetic diversity

    • R. J. Lipshutz, D. Morris, M. Chee, E. Hubbell, M. J. Kozal, N. Shah et al.

    Pages 241-254

11. Generation of libraries by print technologies

    • Eugen Ermantraut, Sefan Wölfl, Hans Peter Saluz

    Pages 255-264

12. Synthesis and screening of bead-based libraries

    • Dirk Vetter

    Pages 265-277

13. Sensors for biomolecular studies

    • Jan Rickert, Thomas Wessa, Wolfgang Göpel

    Pages 279-310

14. Fluorescence detection of single molecules applicable to small volume assays

    • Jörg Enderlein, W. Patrick Ambrose, Peter M. Goodwin, Richard A. Keller

    Pages 311-329

15. Fluorescence correlation spectrometry (FCS): Measuring biological interactions in microstructures

    • Gabriele Gradl, Rolf Guenther, Silvia Sterrer

    Pages 331-351

16. Scanning force microscopy: A microstructured device for imaging, probing, and manipulation of biomolecules at the nanometer scale

    • Wolfgang Fritzsche

    Pages 353-370

17. DNA resequencing, mutation detection and gene expression analysis by oligonucleotide microchips

    • Andres Metspalu, John M. Shumaker

    Pages 371-397

18. Miniaturized arrays for DNA analysis

    • Maryanne J. O’Donnell, Hubert Köster

    Pages 399-415

19. Manipulation of particles, cells and liquid droplets by high frequency electric fields

    • Thomas Schnelle, Torsten Müller, Günter Fuhr

    Pages 417-452

20. Optical trapping and manipulation

    • Karl Otto Greulich

    Pages 453-474

Biomolecular studies are the trial of Man to understand how Nature manages information at the molecular level. The understanding of molecular informa­ tion handling in nature is essential for the molecular optimization in chem­ istry, molecular biology, molecular pharmacology and therefore - as an ex­ ample - for the development of specifically acting drugs. The famous recent method of technical information management is digital electronics. Over the past few years, evidence has arisen that computerized and molecular information managements have many similar and overlapping aspects. For example, both technology and nature use digitized information and both use small structures for the efficient handling of information. Furthermore, they optimize their processes in order to gain a maximum of information with a minimum of invested energy. During the last two decades, novel experimental techniques in biomolec­ ular sciences have paved the way for artificial biomolecular optimization. In the same time interval, the progress of micro system technology has been extended from the field of digital electronics and sensing to micro liquid hand­ ling, and the field of chip-supported substance handling began. It appears that the "marriage" of physical micro technology and molecular processing will be consummated soon. The contact of both fields has been realized in for ex­ ample DNA chips. Such connections will also become relevant in additional fields in the near future. Biomolecular investigations are the first to profit from these fast growing scientific and technical connections between micro systems and molecular sciences.

• Expression
• Nucleotide
• Polymerasekettenreaktion
• biology
• energy
• experiment
• gene expression
• genes
• information
• microscopy
• molecular biology
• peptides
• pharmacology
• protein
• system

• Institut für Physikal, Hochtechnologie Jena, Jena, Germany

  J. M. Köhler

• Jena, Germany

  T. Mejevaia

• Hans-Knöll-Institut für Naturstofforschung e.V., Jena, Germany

  H. P. Saluz

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