Proteomics in Functional Genomics

1. Front Matter

   Pages I-XIII

   PDF

2. Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis

   • Ettore Appella, David Arnott, Kazuyasu Sakaguchi, Peter J. Wirth

   Pages 1-27

3. Techniques for sample preparation including methods for concentrating peptide samples

   • Kris Gevaert, Tony Houthaeve, Joël Vandekerckhove

   Pages 29-42

4. Sample preparation by SDS/PAGE and in-gel digestion

   • Ulf Hellman

   Pages 43-54

5. High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection

   • Julio E. Celis, Pavel Gromov

   Pages 55-67

6. Nanospray mass spectrometry in protein and peptide chemistry

   • William J. Griffiths

   Pages 69-79

7. MALDI-TOF mass spectrometry in protein chemistry

   • Peter Roepstorff

   Pages 81-97

8. The chemistry of protein sequence analysis

   • John E. Shively

   Pages 99-117

9. The alkylated thiohydantoin method for C-terminal sequence analysis

   • David R. Dupont, MeriLisa Bozzini, Victoria L. Boyd

   Pages 119-131

10. Ladder sequencing

    • Tomas Bergman

    Pages 133-144

11. New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer

    • Christian Wurzel, Brigitte Wittmann-Liebold

    Pages 145-157

12. Analysis of glycoprotein-derived glycopeptides

    • Gerrit J. Gerwig, Johannes F. G. Vliegenthart

    Pages 159-186

13. Lipopeptide preparation and analysis

    • Jan Johansson, Margareta Stark, Magnus Gustafsson, Yuqin Wang, Shahparak Zaltash

    Pages 187-198

14. Phosphopeptide analysis

    • Manfredo Quadroni, Peter James

    Pages 199-213

15. Bioinformatics in protein analysis

    • Bengt Persson

    Pages 215-231

16. Back Matter

    Pages 233-236

    PDF

A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

• Polyacrylamid-Gelelektrophorese
• Proteomics
• Western Blotting
• bioinformatics
• chemistry
• databases
• genome
• genomics
• peptides
• protein
• protein sequence
• protein structure
• sequence analysis
• tissue

• Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401, Muséum National d’Histoire Naturelle, Paris, France

  P. Jollès

• Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

  H. Jörnvall

Policies and ethics