Immunofluorescence in Clinical Immunology

1. Front Matter

   Pages I-XVI

   PDF

2. Introduction to the basic principles and techniques of immunofluorescence

   • Wulf B. Storch

   Pages 1-4

3. Production of antibodies and labelling with fluorochromes

   • Wulf B. Storch

   Pages 5-17

4. Characterisation and quality control of antisera and conjugates

   • Wulf B. Storch

   Pages 19-33

5. Actual immunofluorescence techniques

   • Wulf B. Storch

   Pages 35-46

6. Instrumental techniques and the documentation of findings

   • Wulf B. Storch

   Pages 47-56

7. General advice

   • Wulf B. Storch

   Pages 57-58

8. Applications of immunofluorescence

   • Wulf B. Storch

   Pages 59-195

9. Back Matter

   Pages 197-231

   PDF

Immunofluorescence, a suitable laboratory method for the microscopic demonstration of antigens and antibodies in biological materials, useable, for example, to provide evidence for the pathogenesis of disease in histological or cytological preparations and for tumour cell differentiation. For this reason immunofluorescence has a decisive role as the method of choice for the diagnosis of auto-immune diseases. This primer on immunofluorescence techniques, which first appeared in 1979, is a richly illustrated handbook suitable for everyday practical work in the laboratory, useable as both an introduction to the subject as well as an atlas. In hardly any other area of medicine are there so many new findings to report. The second edition of this book is concerned not only with the detection methods which now form an essential and established part of diagnostic techniques, but also with the most recent research results such as the discovery of antibodies against Auerbach's plexus and against podocytes...

• Antigen
• Biologie
• Klinische Immunologie
• Laboratory
• Medizin
• biological materials
• diagnosis
• fluorescence

• Weinheim, Germany

  Wulf B. Storch

Policies and ethics