Skip to main content
SpringerLink
Log in
Birkhäuser

A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions

  • Book
  • © 1991

Overview

Editors:
  1. Jean-Pierre Jost

    1. Friedrich Miescher-Institut, Basel, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Hans-Peter Saluz

    1. IRBM, Pomezia (Rome), Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Biomethods (BIOMETHODS)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 chapters)

  1. Front Matter

    Pages 1-14
    Download chapter PDF

  2. Introduction

    1. Introduction

      • J. P. J., H. P. S.
      Pages 15-17

  3. The Study of Protein-DNA Interactions by Means of Enzymes

    1. The Study of Protein-DNA Interactions by Deoxyribonuclease I Footprinting

      • Dietmar von der Ahe
      Pages 19-34

    2. Exonuclease III Protection Assay for Specific DNA-Binding Proteins

      • Jean-Pierre Jost
      Pages 35-43

    3. The Proteolytic Clipping Band-Shift Assay of Protein-DNA Complexes

      • Melya J. Hughes, Haimin Liang, Jean-Pierre Jost
      Pages 45-54

  5. The Study of Protein-DNA Interactions by Means of Chemicals (Interference Assays)

    1. Interaction of Proteins with Partially Depurinated and Depyrimidinated Oligonucleotides (Missing-Contact Probing of Protein-DNA Interactions)

      • Haimin Liang, André Pawlak, Jean-Pierre Jost
      Pages 93-109

    2. The Study of Protein-DNA Contacts by Ethylation Interference

      • Vichien Rimphanitchayakit, Nigel D. F. Grindley
      Pages 111-120

    3. The Study of Protein-DNA Interactions by Methylation Interference

      • Josef Jiricny, Natalie Corman
      Pages 121-131

    4. Using the Chemistry of the Hydroxyl Radical to Determine Structural Details about DNA and Protein-DNA Complexes

      • Judith R. Levin, Amanda M. Burkhoff, Thomas D. Tullius
      Pages 133-144

    5. Detection of Unusual DNA Structures and DNA-Protein Interactions by Diethylpyrocarbonate Carbethoxylation

      • Miklos Toth
      Pages 145-152

  6. The Study of Protein-DNA Interactions by Means of Electron Microscopy

    1. Electron Microscope Visualisation of Protein-DNA Complexes

      • Béatrice ten Heggeler-Bordier, Walter Wahli
      Pages 153-161

  7. The Study of Protein-DNA Interactions by Gel Mobility-shift Assays

    1. Qualitative and Quantitative Studies of Protein-DNA Interactions by Gel Mobility-Shift Assay

      • Stuart R. Stone, Melya J. Hughes, Jean-Pierre Jost
      Pages 163-194

    2. Diagonal Gel Mobility-Shift Assays for the Resolution of Multi-Subunit Complexes Binding to Regulatory Elements of Specific Genes

      • Fred Schaufele, Brian L. West, Jean-Pierre Jost, Tim Reudelhuber
      Pages 195-206

    3. A Rapid Gel-Shift Technique to Analyse DNA-Protein Interactions using Phastsystem™

      • Ponnusamy Ramanujam, Scott Fogerty, William Heiser, James Jolly
      Pages 207-219

  8. Protein-DNA Interactions on a Solid Support

    1. Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography

      • Melya J. Hughes, Jean-Pierre Jost, Josef Jiricny
      Pages 221-231

    2. Cloning of Sequence-Specific DNA-Binding Proteins by Screening λ cDNA Expression Libraries with Radiolabelled Binding-Site Probes

      • Patrick Matthias, Michael M. Müller, Walter Schaffner
      Pages 233-244

  9. The Study of Protein-DNA Interaction Using Special Vectors

    1. Detection of DNA Bending by Gel Electrophoresis: Use of Plasmid Vectors

      • Christian Zwieb, Jin Kim, Sankar Adhya
      Pages 245-257
Back to top

Keywords

About this book

A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind­ ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.

Editors and Affiliations

  • Friedrich Miescher-Institut, Basel, Switzerland

    Jean-Pierre Jost

  • IRBM, Pomezia (Rome), Italy

    Hans-Peter Saluz

Bibliographic Information

  • Book Title: A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions

  • Editors: Jean-Pierre Jost, Hans-Peter Saluz

  • Series Title: Biomethods

  • DOI: https://doi.org/10.1007/978-3-0348-7561-5

  • Publisher: Birkhäuser Basel

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Verlag Basel 1991

  • Softcover ISBN: 978-3-7643-2627-2Published: 01 September 1991

  • eBook ISBN: 978-3-0348-7561-5Published: 13 March 2013

  • Edition Number: 1

  • Number of Pages: 328

  • Number of Illustrations: 52 b/w illustrations

  • Topics: Science, Humanities and Social Sciences, multidisciplinary

Publish with us

Policies and ethics

Back to top

Search

Navigation