Name: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
ISBN: 978-3-0348-7349-9

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

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Editors:

Thomas Meier⁰,
Falk Fahrenholz¹

Thomas Meier
1. Physiologisches Institut, Universität Basel, Basel, Switzerland
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Falk Fahrenholz
1. Max-Planck-Institut für Biophysik, Frankfurt, Germany
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Part of the book series: Biomethods (BIOMETHODS)

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20 Citations

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Table of contents (14 chapters)

Front Matter

Pages I-XIV

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An Introduction to Avidin-Biotin Technology and Options for Biotinylation
- M. Dean Savage
Pages 1-29
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Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography
- Christoph Thiele, Falk Fahrenholz
Pages 31-44
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Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands
- Tetsuya Ohtaki, Chieko Kitada, Haruo Onda
Pages 45-63
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Photoreactive Biotinylated Peptide Ligands for Affinity Labeling
- M. Fabry, D. Brandenburg
Pages 65-81
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Immunoprecipitation of Biotinylated Cell Surface Proteins
- Thomas Meier, Hermann Leying
Pages 83-97
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Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules
- Joseph G. Altin
Pages 99-114
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Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting
- Elwira Lisowska, Maria Duk, Albert M. Wu
Pages 115-129
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Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions
- Frank M. Narendja, Georg Sauermann
Pages 131-141
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Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinase Inhibitory Proteins
- James Melrose, Kenneth J. Rodgers
Pages 143-165
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Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for in vivo Immunoerythrocyte Exploration
- Vladimir R. Muzykantov
Pages 167-182
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Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin Labeled Proteins in a Cell-Free System
- Hans-Joachim Hoeltke, Irene Ettl, Edith Strobel, Hermann Leying, Maria Zimmermann, Richard Zimmermann
Pages 183-199
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Nonradioactive Detection of Nucleic Acids with Biotinylated Probes
- Rainer Löw, Thomas Rausch
Pages 201-213
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Biotin-Labeled Riboprobes to Study RNA-Binding Proteins
- Pedro L. Rodriguez, Luis Carrasco
Pages 215-225
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Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers
- Ricardo Fujita, Anand Swaroop
Pages 227-237
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Back Matter

Pages 238-240

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Keywords

Editors and Affiliations

Physiologisches Institut, Universität Basel, Basel, Switzerland

Thomas Meier
Max-Planck-Institut für Biophysik, Frankfurt, Germany

Falk Fahrenholz

Bibliographic Information

Book Title: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Editors: Thomas Meier, Falk Fahrenholz
Series Title: Biomethods
DOI: https://doi.org/10.1007/978-3-0348-7349-9
Publisher: Birkhäuser Basel
eBook Packages: Springer Book Archive
Softcover ISBN: 978-3-0348-7351-2Published: 19 March 2012
eBook ISBN: 978-3-0348-7349-9Published: 06 December 2012
Edition Number: 1
Number of Pages: XIV, 240
Topics: Life Sciences, general, Biomedicine general

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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

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Table of contents (14 chapters)

Front Matter

An Introduction to Avidin-Biotin Technology and Options for Biotinylation

Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography

Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands

Photoreactive Biotinylated Peptide Ligands for Affinity Labeling

Immunoprecipitation of Biotinylated Cell Surface Proteins

Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules

Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting

Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions

Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinase Inhibitory Proteins

Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for in vivo Immunoerythrocyte Exploration

Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin Labeled Proteins in a Cell-Free System

Nonradioactive Detection of Nucleic Acids with Biotinylated Probes

Biotin-Labeled Riboprobes to Study RNA-Binding Proteins

Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers

Back Matter

Keywords

Editors and Affiliations

Physiologisches Institut, Universität Basel, Basel, Switzerland

Max-Planck-Institut für Biophysik, Frankfurt, Germany

Bibliographic Information

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Table of contents (14 chapters)

Front Matter

Back Matter

Keywords

Editors and Affiliations

Physiologisches Institut, Universität Basel, Basel, Switzerland

Max-Planck-Institut für Biophysik, Frankfurt, Germany

Bibliographic Information

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Buy it now

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