Editors:
Comprehensive guide to all techniques required for the generation of classical and conditional knockout mouse strains
Includes techniques for ES cell establishment, ES cell in vitro differentatio, and nuclear cloning
Provides a systematic overview of techniques for mutant phenotyping
Covers all gene knockout techniques, including gene targeting, chemical, gene trap, and transposon mutagenesis
Written by hands-on experts who have optimized each method for experimental success
Part of the book series: Methods in Molecular Biology (MIMB, volume 530)
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Table of contents (25 protocols)
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Front Matter
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Gene Modification in ES Cells
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Front Matter
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Stem Cell Manipulation
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Front Matter
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Genetically Engineered Mice
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Front Matter
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About this book
Reviews
From the reviews of the second edition:
"This comprehensive collection of step-by-step protocols in the field of gene targeting and mouse genetics covers such topics as gene modification in embryonic stem cells … and mutant phenotype analysis. … The book will serve as a valuable resource for researchers, scientists, and students interested in these areas. … This is a well-illustrated book … . All researchers interested in gene targeting and gene knockout protocols will find this extremely useful." (Omer Iqbal, Doody’s Review Service, September, 2009)
Bibliographic Information
Book Title: Gene Knockout Protocols
Editors: Wolfgang Wurst, Ralf Kühn
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-471-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-26-8Published: 27 March 2009
Softcover ISBN: 978-1-62703-834-8Published: 30 October 2014
eBook ISBN: 978-1-59745-471-1Published: 06 March 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 516
Topics: Biochemistry, general, Human Genetics, Animal Genetics and Genomics, Animal Models