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Monoclonal Antibodies

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Vincent Ossipow

    1. Département de Biochimie, University of Geneva, Geneva, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  2. Nicolas Fischer

    1. NovImmune SA, Plan-les-Ouates, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Offers modern approaches to generate high quality monoclonals
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1131)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Antibody Generation

    1. Front Matter

      Pages 1-1
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    2. Antigen Production for Monoclonal Antibody Generation

      • Giovanni Magistrelli, Pauline Malinge
      Pages 3-20

    3. Method for the Generation of Antibodies Specific for Site and Posttranslational Modifications

      • Hidemasa Goto, Masaki Inagaki
      Pages 21-31

    4. Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production

      • Hyung-Yong Kim, Alexander Stojadinovic, Mina J. Izadjoo
      Pages 33-45

    5. Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion

      • Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Cindi R. Corbett, Jody D. Berry
      Pages 47-70

    6. Generation of Rabbit Monoclonal Antibodies

      • Pi-Chen Yam, Katherine L. Knight
      Pages 71-79

    7. Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry

      • Alejandro Uribe-Benninghoff, Teresa Cabral, Efthalia Chronopoulou, Jody D. Berry, Cindi R. Corbett
      Pages 81-103

    8. Screening and Subcloning of High Producer Transfectomas Using Semisolid Media and Automated Colony Picker

      • Suba Dharshanan, Cheah Swee Hung
      Pages 105-112

    9. Design and Generation of Synthetic Antibody Libraries for Phage Display

      • Gang Chen, Sachdev S. Sidhu
      Pages 113-131

    10. Selection and Screening Using Antibody Phage Display Libraries

      • Patrick Koenig, Germaine Fuh
      Pages 133-149

    11. Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening, and Affinity Maturation

      • James A. Van Deventer, Karl Dane Wittrup
      Pages 151-181

    12. Human B Cell Immortalization for Monoclonal Antibody Production

      • Joyce Hui-Yuen, Siva Koganti, Sumita Bhaduri-McIntosh
      Pages 183-189

    13. Using Next-Generation Sequencing for Discovery of High-Frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice

      • Ulrike Haessler, Sai T. Reddy
      Pages 191-203

  3. Antibody Expression and Purification

    1. Front Matter

      Pages 205-205
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    2. Cloning, Reformatting, and Small-Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma

      • Jeremy Loyau, François Rousseau
      Pages 207-228

    3. Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid

      • Nicole Müller-Sienerth, Cécile Crosnier, Gavin J. Wright, Nicole Staudt
      Pages 229-240

    4. Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography

      • Larry J. Cummings, Russell G. Frost, Mark A. Snyder
      Pages 241-251

    5. Rapid Purification of Monoclonal Antibodies Using Magnetic Microspheres

      • Pauline Malinge, Giovanni Magistrelli
      Pages 253-261

    6. Generation of Cell Lines for Monoclonal Antibody Production

      • Krista Alvin, Jianxin Ye
      Pages 263-271
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Keywords

About this book

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

Editors and Affiliations

  • Département de Biochimie, University of Geneva, Geneva, Switzerland

    Vincent Ossipow

  • NovImmune SA, Plan-les-Ouates, Switzerland

    Nicolas Fischer

Bibliographic Information

  • Book Title: Monoclonal Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Vincent Ossipow, Nicolas Fischer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-992-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-991-8Published: 12 February 2014

  • Softcover ISBN: 978-1-4939-6322-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-992-5Published: 10 February 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 575

  • Number of Illustrations: 81 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology, Laboratory Medicine, Antibodies

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