Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1129)
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Table of contents (38 protocols)
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Front Matter
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Screening and Design Purification Strategies
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Front Matter
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Low-Resolution Protein Purification Methods
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Front Matter
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High-Resolution Protein Purification Methods
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Front Matter
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Keywords
About this book
Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.
Editors and Affiliations
Bibliographic Information
Book Title: Protein Downstream Processing
Book Subtitle: Design, Development and Application of High and Low-Resolution Methods
Editors: Nikolaos E. Labrou
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-977-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-976-5Published: 20 March 2014
Softcover ISBN: 978-1-4939-6040-8Published: 03 September 2016
eBook ISBN: 978-1-62703-977-2Published: 19 March 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 555
Number of Illustrations: 59 b/w illustrations, 62 illustrations in colour
Topics: Protein Science