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  • Book
  • © 2014

Polyadenylation

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1125)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
  2. 3′-End Processing in Eukaryotes

    1. Front Matter

      Pages 1-1
    2. 3′ End Formation and Regulation of Eukaryotic mRNAs

      • Thomas J. Sweet, Donny D. Licatalosi
      Pages 3-12
    3. Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay

      • Deepak P. Patil, Baskar Bakthavachalu, Daniel R. Schoenberg
      Pages 13-23
    4. Using Klenow-Mediated Extension to Measure Poly(A)-Tail Length and Position in the Transcriptome

      • Man Chun Lee, Amrei Jänicke, Traude Helene Beilharz
      Pages 25-42
    5. Detection of Uridylated mRNAs

      • François M. Sement, Dominique Gagliardi
      Pages 43-51
    6. Cytoplasmic Polyadenylation Assays

      • Olga Coll, Ana Villalba, Fátima Gebauer
      Pages 53-63
    7. In Vitro Analysis of Transcriptional Activators and Polyadenylation

      • Takashi Nagaike, James L. Manley
      Pages 65-74
    8. Small Molecule Recognition of Poly(A)

      • Özgül Persil Çetinkol
      Pages 81-108
    9. Polyadenylation Site Prediction Using PolyA-iEP Method

      • Ioannis Kavakiotis, George Tzanis, Ioannis Vlahavas
      Pages 131-140
    10. Detecting Alternative Polyadenylation from Microarray Data

      • Antonio Lembo, Paolo Provero
      Pages 141-144
    11. RHAPA: A New Method to Quantify Alternative Polyadenylation

      • Ashley L. Cornett, Carol S. Lutz
      Pages 157-167
    12. Multiplex Analysis of PolyA-Linked Sequences (MAPS): An RNA-Seq Strategy to Profile Poly(A+) RNA

      • Yu Zhou, Hai-Ri Li, Jie Huang, Ge Jin, Xiang-Dong Fu
      Pages 169-178
  3. Bacteria and Organelles

    1. Front Matter

      Pages 209-209

About this book

In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • MRC Mitochondrial Biology Unit, Mitochondrial Genetics Group, Cambridge, United Kingdom

    Joanna Rorbach

  • Mitochondrial Research Group, Newcastle University The Medical School, Newcastle upon Tyne, United Kingdom

    Agnieszka J. Bobrowicz

Bibliographic Information

  • Book Title: Polyadenylation

  • Book Subtitle: Methods and Protocols

  • Editors: Joanna Rorbach, Agnieszka J. Bobrowicz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-971-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-970-3Published: 04 March 2014

  • Softcover ISBN: 978-1-4939-6055-2Published: 03 September 2016

  • eBook ISBN: 978-1-62703-971-0Published: 28 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 377

  • Number of Illustrations: 41 b/w illustrations, 22 illustrations in colour

  • Topics: Human Genetics, Gene Function

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access