Editors:
- Provides neutrophil protocols that are broadly applicable to leukocyte biology
- Includes protocols for isolation from humans and animal species
- Presents an interesting and informative overview of the field of neutrophil biology
- Facilitates a better understanding of the role of neutrophils in host defense
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1124)
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Table of contents (32 protocols)
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Front Matter
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Neutrophils: An Overview
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Front Matter
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Neutrophil Isolation and Subcellular Fractionation
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Front Matter
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Biochemistry, Biology and Signal Transduction of Neutrophils
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Front Matter
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About this book
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
Editors and Affiliations
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Montana State University Dept. Veterinary Molecular Biology, Bozeman, USA
Mark T. Quinn
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Lab. Human Bacterial Pathogenesis, National Institutes of Health Nat. Inst. Allergy & Infectious Diseases, Hamilton, USA
Frank R. DeLeo
Bibliographic Information
Book Title: Neutrophil Methods and Protocols
Editors: Mark T. Quinn, Frank R. DeLeo
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-845-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-844-7Published: 07 February 2014
Softcover ISBN: 978-1-4939-6062-0Published: 27 August 2016
eBook ISBN: 978-1-62703-845-4Published: 07 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 551
Number of Illustrations: 65 b/w illustrations, 28 illustrations in colour
Topics: Infectious Diseases, Microbial Genetics and Genomics