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Bioluminescent Imaging

Methods and Protocols

  • Book
  • © 2014

Overview

  • Includes cutting-edge methods and protocols to examine bioluminescent imaging
  • Provides step-by-step, easy to follow detail essential for reproduction
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1098)

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Table of contents (20 protocols)

  1. In Vitro Bioassays

  2. Imaging Molecular and Cellular Events

  3. Imaging of Disease (Pathogenesis)

  4. Therapeutic Imaging

Keywords

About this book

Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.

Reviews

From the reviews:

“The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. … The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields.” (Omer Iqbal, Doody's Book Reviews, January, 2014)

Editors and Affiliations

  • Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, USA

    Christian E. Badr

Bibliographic Information

  • Book Title: Bioluminescent Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Christian E. Badr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-718-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-717-4Published: 29 October 2013

  • Softcover ISBN: 978-1-4939-6067-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-718-1Published: 28 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 276

  • Number of Illustrations: 11 b/w illustrations, 39 illustrations in colour

  • Topics: Imaging / Radiology

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