Editors:
- Seeks to widen the view on miRNA as biological mediator and potential drug target
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1095)
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Table of contents (15 protocols)
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Front Matter
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Methods
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Front Matter
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Back Matter
About this book
In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
Keywords
- Dicer-mediated miRNA maturation
- Drosha activity
- PNA as anti-miRs
- biological mediator
- fluorescent methods
- manipulate miRNA activity
- miRNA maturation inhibitors
- non-fluorescent methods
- oligonucleotide approaches
- potential drug target
- qRT-PCR of miRNA maturation components
- small molecule miRNA effectors
- gene expression
Editors and Affiliations
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Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Germany
Christoph Arenz
Bibliographic Information
Book Title: miRNA Maturation
Book Subtitle: Methods and Protocols
Editors: Christoph Arenz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-703-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-62703-702-0Published: 29 October 2013
Softcover ISBN: 978-1-4939-6016-3Published: 23 August 2016
eBook ISBN: 978-1-62703-703-7Published: 28 October 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 192
Number of Illustrations: 21 b/w illustrations, 29 illustrations in colour
Topics: Human Genetics, Gene Expression