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Structural Genomics

General Applications

  • Book
  • © 2014

Overview

Editors:
  1. Yu Wai Chen

    1. King's College London, London, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1091)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
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  2. Cloning, Expression and Protein Production

    1. Front Matter

      Pages 1-1
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    2. DisMeta: A Meta Server for Construct Design and Optimization

      • Yuanpeng Janet Huang, Thomas B. Acton, Gaetano T. Montelione
      Pages 3-16

    3. Stable Expression Clones and Auto-Induction for Protein Production in E. coli

      • F. William Studier
      Pages 17-32

    4. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli

      • Natalie J. Saez, Renaud Vincentelli
      Pages 33-53

    5. Medium-Throughput Production of Recombinant Human Proteins: Ligation-Independent Cloning

      • Claire Strain-Damerell, Pravin Mahajan, Opher Gileadi, Nicola A. Burgess-Brown
      Pages 55-72

    6. Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli

      • Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, Susanne Gräslund
      Pages 73-94

    7. Medium-Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells

      • Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, Nicola A. Burgess-Brown
      Pages 95-121

    8. OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems

      • Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, Imre Berger
      Pages 123-130

    9. Multiprotein Complex Production in Insect Cells by Using Polyproteins

      • Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, Imre Berger
      Pages 131-141

    10. Expression Screening in Mammalian Suspension Cells

      • Susan D. Chapple, Michael R. Dyson
      Pages 143-149

    11. Cell-Free Expression of Protein Complexes for Structural Biology

      • Takaho Terada, Takeshi Murata, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 151-159

    12. Cell-Free Protein Synthesis for Functional and Structural Studies

      • Shin-ichi Makino, Emily T. Beebe, John L. Markley, Brian G. Fox
      Pages 161-178

    13. Insoluble Protein Purification with Sarkosyl: Facts and Precautions

      • Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, Yu Wai Chen
      Pages 179-186

  3. Experimental Structure Determination and Characterization

    1. Front Matter

      Pages 187-187
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    2. Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay

      • Vincent Mariaule, Florine Dupeux, José A. Márquez
      Pages 189-195

    3. CrystalDirect™: A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films

      • José A. Márquez, Florent Cipriani
      Pages 197-203

    4. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy

      • Michael R. Sawaya
      Pages 205-214

    5. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination

      • Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, Michael A. Kennedy
      Pages 215-227

    6. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labeled Mutants for NMR Studies of High Molecular Weight Proteins

      • Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet et al.
      Pages 229-244
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Keywords

About this book

The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.

Editors and Affiliations

  • King's College London, London, United Kingdom

    Yu Wai Chen

Bibliographic Information

  • Book Title: Structural Genomics

  • Book Subtitle: General Applications

  • Editors: Yu Wai Chen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-691-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-690-0Published: 09 November 2013

  • Softcover ISBN: 978-1-4939-6060-6Published: 23 August 2016

  • eBook ISBN: 978-1-62703-691-7Published: 08 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 358

  • Number of Illustrations: 17 b/w illustrations, 63 illustrations in colour

  • Topics: Human Genetics, Proteomics

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