Skip to main content
SpringerLink
Log in
Book cover

Exocytosis Methods

  • Book
  • © 2014

Overview

Editors:
  1. Peter Thorn

    1. School of Biomedical Sciences, University of Queensland, St. Lucia, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques and methods for studying exocytosis in the brain
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 83)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (11 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Techniques in the Measurement of Exocytosis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating-Laser Excitation

      • Jae-Yeol Kim, Bong-Kyu Choi, Yeon-Kyun Shin, Nam Ki Lee
      Pages 3-22

    3. Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes

      • Thomas Haller, Paul Dietl
      Pages 23-37

    4. Carbon-Fiber Amperometry in the Study of Exocytosis

      • Michael D. Duffield, Ravinarayan Raghupathi, Damien J. Keating
      Pages 39-54

    5. Imaging of Insulin Exocytosis from Pancreatic Beta Cells

      • Mica Ohara-Imaizumi, Kyota Aoyagi, Shinya Nagamatsu
      Pages 55-74

    6. Functional, Quantitative, and Super-Resolution Imaging and Spectroscopic Approaches for Studying Exocytosis

      • Rory R. Duncan, Colin Rickman
      Pages 75-91

    7. Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells

      • BoÅ¡tjan Rituper, Robert Zorec
      Pages 93-123

  3. Model Systems for the Study of Exocytosis

    1. Front Matter

      Pages 125-125
      Download chapter PDF

    2. Measuring Exocytosis in Endocrine Tissue Slices

      • MaÅ¡a Skelin Klemen, Jurij DolenÅ¡ek, Andraž Stožer, Marjan Slak Rupnik
      Pages 127-146

    3. Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents

      • Oleg Milberg, Natalie Porat-Shliom, Muhibullah Tora, Laura Parente, Andrius Masedunskas, Roberto Weigert
      Pages 147-166

    4. Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomes

      • James A. Daniel, Phillip J. Robinson
      Pages 167-189

    5. Isolated Neurohypophysial Terminals: Model for Depolarization–Secretion Coupling

      • José R. Lemos, James McNally, Edward Custer, Adolfo Cuadra, Hector Marrero, Dixon Woodbury
      Pages 191-220

    6. The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca2+-Triggered Steps of Regulated Exocytosis

      • Prabhodh S. Abbineni, Elise P. Wright, Tatiana P. Rogasevskaia, Murray C. Killingsworth, Chandra S. Malladi, Jens R. Coorssen
      Pages 221-241

  4. Back Matter

    Pages 243-248
    Download chapter PDF
Back to top

Keywords

About this book

Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.  The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.  The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.  Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results.

 

Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field.

Editors and Affiliations

  • School of Biomedical Sciences, University of Queensland, St. Lucia, Australia

    Peter Thorn

Bibliographic Information

  • Book Title: Exocytosis Methods

  • Editors: Peter Thorn

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-62703-676-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-675-7Published: 01 November 2013

  • Softcover ISBN: 978-1-4939-6329-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-676-4Published: 31 October 2013

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 248

  • Number of Illustrations: 34 b/w illustrations, 36 illustrations in colour

  • Topics: Neurosciences, Neurobiology

Publish with us

Policies and ethics

Back to top

Search

Navigation