Editors:
- Follows the step-by-step life cycle of a retrovirus
- Provides state-of-the-art methodological protocols from world leaders in human retrovirology
- Includes expert notes on troubleshooting and avoiding known pitfalls
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1087)
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Table of contents (27 protocols)
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Front Matter
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Mapping the HIV Life Cycle
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Front Matter
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Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
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Front Matter
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About this book
Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore “assay and function of accessory genes”, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.
Editors and Affiliations
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Div. Immunology, Transplantation, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy
Elisa Vicenzi, Guido Poli
Bibliographic Information
Book Title: Human Retroviruses
Book Subtitle: Methods and Protocols
Editors: Elisa Vicenzi, Guido Poli
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-670-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-669-6Published: 25 October 2013
Softcover ISBN: 978-1-4939-6258-7Published: 23 August 2016
eBook ISBN: 978-1-62703-670-2Published: 25 October 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 345
Number of Illustrations: 24 b/w illustrations, 38 illustrations in colour
Topics: Immunology, Virology