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  • © 2014

Brain Development

Methods and Protocols

  • Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 1082)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
  2. Molluscs

    1. Front Matter

      Pages 115-115
    2. Methods in Brain Development of Molluscs

      • Andreas Wanninger, Tim Wollesen
      Pages 117-125
  3. Xenopus Protocols

    1. Front Matter

      Pages 127-127
    2. In Situ Hybridization and Immunostaining of Xenopus Brain

      • Kai-li Liu, Xiu-mei Wang, Zi-long Li, Rong-qiao He, Ying Liu
      Pages 129-141
    3. Microinjection Manipulations in the Elucidation of Xenopus Brain Development

      • Cristine Smoczer, Lara Hooker, Saqib S. Sachani, Michael J. Crawford
      Pages 143-154
    4. Morpholino Studies in Xenopus Brain Development

      • Jennifer E. Bestman, Hollis T. Cline
      Pages 155-171
  4. Zebrafish Protocols

    1. Front Matter

      Pages 173-173
    2. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish

      • Adèle Faucherre, Hernán López-Schier
      Pages 187-195
    3. Anatomical Dissection of Zebrafish Brain Development

      • Katherine J. Turner, Thomas G. Bracewell, Thomas A. Hawkins
      Pages 197-214

About this book

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.

Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G Sprecher

Bibliographic Information

  • Book Title: Brain Development

  • Book Subtitle: Methods and Protocols

  • Editors: Simon G Sprecher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-655-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-654-2Published: 19 September 2013

  • Softcover ISBN: 978-1-4939-6025-5Published: 29 September 2016

  • eBook ISBN: 978-1-62703-655-9Published: 19 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 317

  • Number of Illustrations: 14 b/w illustrations, 49 illustrations in colour

  • Topics: Neurosciences, Neurobiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access