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  • Book
  • © 2014

Plant Cell Morphogenesis

Methods and Protocols

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1080)

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Softcover Book USD 119.00
Price excludes VAT (USA)
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Hardcover Book USD 109.99
Price excludes VAT (USA)
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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. Image Analysis: Basic Procedures for Description of Plant Structures

    • Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, Jiří Janáček
    Pages 67-76
  3. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces

    • Dorota Kwiatkowska, Agata Burian
    Pages 99-110
  4. Time-Lapse Imaging of Developing Meristems Using Confocal Laser Scanning Microscope

    • Olivier Hamant, Pradeep Das, Agata Burian
    Pages 111-119
  5. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem Using MorphoGraphX

    • Pierre Barbier de Reuille, Sarah Robinson, Richard S. Smith
    Pages 121-134
  6. Immunogold Labeling of Resin-Embedded Electron Microscopical Sections

    • Ilse Foissner, Margit Hoeftberger
    Pages 183-193
  7. Morphological Analysis of Cell Growth Mutants in Physcomitrella

    • Jeffrey P. Bibeau, Luis Vidali
    Pages 201-213
  8. Plant Cell Lines in Cell Morphogenesis Research

    • Daniela Seifertová, Petr Klíma, Markéta Pařezová, Jan Petrášek, Eva Zažímalová, Zdeněk Opatrný
    Pages 215-229
  9. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

    • Radek Bezvoda, Roman Pleskot, Viktor Žárský, Martin Potocký
    Pages 231-236

About this book

 Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on  classical light microscopy  and  histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized  electron microscopy  techniques, and methods  used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell  morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by  expert researchers in the field and  include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Reviews

From the book reviews:

“A compendium of procedures and techniques for investigating the structure and shape of plant tissues and cells in a developmental context. … I can certainly rate this book very useful for the scientific community, as it contains many valuable new techniques and inside knowledge. Furthermore, it could inspire researchers to use new technical approaches for their research topics and discuss these with the expert authors of these articles.” (Kai R. Konrad, Journal of Plant Physiology, Vol. 171, 2014)

Editors and Affiliations

  • Laboratory of Cell Biology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Viktor Žárský

  • Faculty of Science, Charles University, Prague, Czech Republic

    Fatima Cvrčková

Bibliographic Information

  • Book Title: Plant Cell Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Viktor Žárský, Fatima Cvrčková

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-643-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-642-9Published: 17 October 2013

  • Softcover ISBN: 978-1-4939-6032-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-643-6Published: 17 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 10 b/w illustrations, 33 illustrations in colour

  • Topics: Plant Sciences, Plant Physiology

Buy it now

Buying options

Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access