Editors:
- Presents detailed protocols for sirtuin research that can be followed directly or modified to explore new areas of study
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1077)
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Table of contents (21 protocols)
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Front Matter
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Methods to Generate Sirtuin Biology Tools
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Front Matter
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Methods to Identify Sirtuin Substrates
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Front Matter
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Methods to Measure Sirtuin Activity
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Front Matter
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Methods to Study Sirtuin Biology
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Front Matter
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About this book
Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.Editors and Affiliations
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Duke University Medical Center Institute of Molecular Physiology, Durham, USA
Matthew D. Hirschey
Bibliographic Information
Book Title: Sirtuins
Book Subtitle: Methods and Protocols
Editors: Matthew D. Hirschey
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-637-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-636-8Published: 07 September 2013
Softcover ISBN: 978-1-4939-6045-3Published: 23 August 2016
eBook ISBN: 978-1-62703-637-5Published: 06 September 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 314
Number of Illustrations: 33 b/w illustrations, 36 illustrations in colour
Topics: Animal Genetics and Genomics, Proteomics