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Sirtuins

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Matthew D. Hirschey

    1. Duke University Medical Center Institute of Molecular Physiology, Durham, USA

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  • Presents detailed protocols for sirtuin research that can be followed directly or modified to explore new areas of study
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1077)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Methods to Generate Sirtuin Biology Tools

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Introduction: Sirtuins in Aging and Diseases

      • Leonard Guarente
      Pages 3-10

    3. Sirtuins in Yeast: Phenotypes and Tools

      • Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, Brian K. Kennedy
      Pages 11-37

    4. C. elegans Sirtuins

      • Mohan Viswanathan, Heidi A. Tissenbaum
      Pages 39-56

    5. Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster

      • Jason G. Wood, Rachel Whitaker, Stephen L. Helfand
      Pages 57-67

    6. Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis

      • Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, Kristin A. Anderson
      Pages 69-78

  3. Methods to Identify Sirtuin Substrates

    1. Front Matter

      Pages 79-79
      Download chapter PDF

    2. Mass Spectrometry-Based Detection of Protein Acetylation

      • Yu Li, Jeffrey C. Silva, Mary E. Skinner, David B. Lombard
      Pages 81-104

    3. SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome

      • Yue Chen, Gozde Colak, Yingming Zhao
      Pages 105-120

    4. Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry

      • Matthew J. Rardin, Jason M. Held, Bradford W. Gibson
      Pages 121-131

    5. Identification of Deacetylase Substrates with the Biotin Switch Approach

      • J. Will Thompson, Alex Robeson, Joshua L. Andersen
      Pages 133-148

    6. Assaying Chromatin Sirtuins

      • Lei Zhong, Barbara Martinez-Pastor, Dafne M. Silberman, Carlos Sebastian, Raul Mostoslavsky
      Pages 149-163

  5. Methods to Study Sirtuin Biology

    1. Front Matter

      Pages 239-239
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Keywords

About this book

Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.

Editors and Affiliations

  • Duke University Medical Center Institute of Molecular Physiology, Durham, USA

    Matthew D. Hirschey

Bibliographic Information

  • Book Title: Sirtuins

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew D. Hirschey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-637-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-636-8Published: 07 September 2013

  • Softcover ISBN: 978-1-4939-6045-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-637-5Published: 06 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 314

  • Number of Illustrations: 33 b/w illustrations, 36 illustrations in colour

  • Topics: Animal Genetics and Genomics, Proteomics

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