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  • © 2013

Epiblast Stem Cells

Methods and Protocols

Editors:

  • Serves as a resource to individuals interested in studying the biology of pluripotent cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1074)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
  2. Goat Embryonic Stem-Like Cell Derivation and Characterization

    • Esmail Behboodi, Louise Lam, William G. Gavin, Alla Bondareva, Ina Dobrinski
    Pages 51-67
  3. Derivation and Culture of Canine Embryonic Stem Cells

    • Ian C. Tobias, Courtney R. Brooks, Jonathan H. Teichroeb, Dean H. Betts
    Pages 69-83
  4. Isolation and Culture of Porcine Embryonic Stem Cells

    • Ivan Vassiliev, Mark B. Nottle
    Pages 85-95
  5. Isolation and Culture of Pig Epiblast Stem Cells

    • Aida Rodriguez, David A. Contreras, Ramiro Alberio
    Pages 97-110
  6. Isolation and Culture of Bovine Embryonic Stem Cells

    • Shanbo Cao, Fang Wang, Lin Liu
    Pages 111-123
  7. Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos

    • Isabelle Hue, Severine A. Degrelle, Christoph Viebahn
    Pages 125-135
  8. Chicken Embryonic Stem Cells: Establishment and Characterization

    • Pauline Aubel, Bertrand Pain
    Pages 137-150
  9. Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

    • Cantas Alev, Mikiharu Nakano, Yuping Wu, Hiroyuki Horiuchi, Guojun Sheng
    Pages 151-173
  10. Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells

    • Mikkel A. Rasmussen, Vanessa J. Hall, Poul Hyttel
    Pages 185-198
  11. Derivation of Neural Precursors from Bovine Preimplantation Embryos

    • Giovanna Lazzari, Silvia Colleoni, Cesare Galli
    Pages 199-207
  12. Back Matter

    Pages 209-216

About this book

In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.  These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.

 

Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.

Editors and Affiliations

  • School of Biosciences Rm 223 South Lab, University of Nottingham, Loughborough, United Kingdom

    Ramiro Alberio

Bibliographic Information

  • Book Title: Epiblast Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Ramiro Alberio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-628-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-627-6Published: 23 August 2013

  • Softcover ISBN: 978-1-4939-6033-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-628-3Published: 23 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 216

  • Number of Illustrations: 15 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology, Stem Cells

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access