Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1071)
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Table of contents (18 protocols)
Keywords
About this book
In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Â Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.
Editors and Affiliations
Bibliographic Information
Book Title: Fluorescent Protein-Based Biosensors
Book Subtitle: Methods and Protocols
Editors: Jin Zhang, Qiang Ni, Robert H Newman
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-622-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-621-4Published: 20 September 2013
Softcover ISBN: 978-1-4939-6014-9Published: 23 August 2016
eBook ISBN: 978-1-62703-622-1Published: 20 September 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 251
Number of Illustrations: 12 b/w illustrations, 48 illustrations in colour
Topics: Protein Science, Cell Physiology