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  • © 2013

Legume Genomics

Methods and Protocols

Editors:

  • Features cutting-edge methods and protocols examining genes and gene interactions in legumes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1069)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. The Model Legume Genomes

    • Steven B. Cannon
    Pages 1-14
  3. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes

    • Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas et al.
    Pages 25-42
  4. RNA-Seq for Transcriptome Analysis in Non-model Plants

    • Rohini Garg, Mukesh Jain
    Pages 43-58
  5. Functional Analysis of Legume Genome Arrays

    • Nicolas Goffard, Georg Weiller
    Pages 59-66
  6. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes

    • Cecilia Contreras-Cubas, Alejandra A. Covarrubias, José Luis Reyes
    Pages 81-92
  7. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines

    • Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, Kirankumar S. Mysore
    Pages 93-100
  8. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection

    • Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu, Ilaria Losini, Pietro Piffanelli et al.
    Pages 101-118
  9. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants

    • Dorian Fabian UrbaÅ„ski, Anna MaÅ‚olepszy, Jens Stougaard, Stig Uggerhøj Andersen
    Pages 119-146
  10. Isolation and Forward Genetic Analysis of Developmental Genes in Pea

    • James L. Weller, Valérie F. G. Hecht, Frances C. Sussmilch
    Pages 147-161
  11. Gene Silencing in Medicago truncatula Roots Using RNAi

    • Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, Maria J. Harrison
    Pages 163-177
  12. Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration

    • Kelsey Picard, Robyn Lee, Roger Hellens, Richard Macknight
    Pages 215-226
  13. Phosphoproteomic Analysis of Peptides

    • Mark A. Baker
    Pages 271-277
  14. Plant Metabolomics: From Experimental Design to Knowledge Extraction

    • Amit Rai, Shivshankar Umashankar, Sanjay Swarup
    Pages 279-312

About this book

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

Editors and Affiliations

  • CILR, The University of Newcastle Environmental & Life Sciences, Callaghan, Australia

    Ray J. Rose

Bibliographic Information

  • Book Title: Legume Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Ray J. Rose

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-613-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-612-2Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6024-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-613-9Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 316

  • Number of Illustrations: 30 b/w illustrations, 23 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Gene Function

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access