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Immunoproteomics

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Kelly M. Fulton

    1. National Research Council Canada Human Health Therapeutics, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Susan M. Twine

    1. National Research Council Canada Human Health Therapeutics, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides current diverse approaches and techniques to study the immunoproteome
  • Offers lab-ready protocols proven and used by experts around the world
  • Features key tips and implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1061)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to the Immune System

    • Scott McComb, Aude Thiriot, Lakshmi Krishnan, Felicity Stark
    Pages 1-20

  3. Immunoproteomics: Current Technology and Applications

    • Kelly M. Fulton, Susan M. Twine
    Pages 21-57

  4. Antigen Identification Using SEREX

    • Ugur Sahin, Özlem Türeci
    Pages 59-77

  5. Antigen Discovery Using Whole-Genome Phage Display Libraries

    • Elisa Beghetto, Nicola Gargano
    Pages 79-95

  6. Methods and Applications of Serological Proteome Analysis

    • Kelly M. Fulton, Shannon S. Martin, Lawrence Wolfraim, Susan M. Twine
    Pages 97-112

  7. Pre-absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins

    • Guangjin Liu, Wei Zhang, Chengping Lu
    Pages 113-121

  8. Identification of the Antigen Content of Electroimmunoprecipitates

    • N. Helena Beyer, Niels H. H. Heegaard
    Pages 123-140

  9. An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus

    • Kevin M. Downard
    Pages 141-153

  10. In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides That Are Circulating During Microbial Infections

    • Sindy J. Chaves, Kathleen Schegg, Thomas R. Kozel, David P. AuCoin
    Pages 155-165

  11. Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies

    • Alexander Nøstdal, Hans H. Wandall
    Pages 167-179

  12. Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures

    • Luc Tessier, Kelly M. Fulton, Susan M. Twine
    Pages 181-195

  13. Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization

    • Richard Ouedraogo, Julien Textoris, Aurélie Daumas, Christian Capo, Jean-Louis Mege
    Pages 197-209

  14. Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes

    • Richard H. Maier, Christina J. Maier, Kamil Önder
    Pages 211-229

  15. Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis

    • Leonia Bozzacco, Haiqiang Yu
    Pages 231-243

  16. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

    • Fei Wen, Huimin Zhao
    Pages 245-264

  17. Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology

    • Greg Harris, Wangxue Chen
    Pages 265-278

  18. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

    • Timothy J. Waybright, King C. Chan, Timothy D. Veenstra, Zhen Xiao
    Pages 279-289

  19. Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry

    • Theodoros A. Koutroukides, Julian A. J. Jaros, Bob Amess, Daniel Martins-de-Souza, Paul C. Guest, Hassan Rahmoune et al.
    Pages 291-307

  20. Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes

    • Simon J. Foote
    Pages 309-322
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Keywords

About this book

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Editors and Affiliations

  • National Research Council Canada Human Health Therapeutics, Ottawa, Canada

    Kelly M. Fulton, Susan M. Twine

Bibliographic Information

  • Book Title: Immunoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Kelly M. Fulton, Susan M. Twine

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-589-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-588-0Published: 21 August 2013

  • Softcover ISBN: 978-1-4939-6314-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-589-7Published: 21 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 370

  • Number of Illustrations: 40 b/w illustrations, 33 illustrations in colour

  • Topics: Immunology, Proteomics

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