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Human Monoclonal Antibodies

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Michael Steinitz

    1. Department of Pathology, The Lautenberg Center, IMRIC The Hebrew University Hadassah Medical School, Jerusalem, Israel

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1060)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Human Monoclonal Antibodies: The Residual Challenge of Antibody Immunogenicity

    • Herman Waldmann
    Pages 1-8

  3. Technical and Ethical Limitations in Making Human Monoclonal Antibodies (An Overview)

    • Mark C. Glassy, Rishab Gupta
    Pages 9-36

  4. Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases

    • Sotirios Kotsovilis, Evangelos Andreakos
    Pages 37-59

  5. Therapeutic Human Monoclonal Antibodies Against Cancer

    • Jamie Jarboe, Anumeha Gupta, M. Wasif Saif
    Pages 61-77

  6. Polyclonal and Monoclonal Antibodies in Clinic

    • Bharath Wootla, Aleksandar Denic, Moses Rodriguez
    Pages 79-110

  7. Production of Human Monoclonal Antibodies by the Epstein–Barr Virus Method

    • Michael Steinitz
    Pages 111-122

  8. Humanization and Simultaneous Optimization of Monoclonal Antibody

    • T. Kuramochi, T. Igawa, H. Tsunoda, K. Hattori
    Pages 123-137

  9. Chimeric Antibodies

    • Kohei Kurosawa, Waka Lin, Kunihiro Ohta
    Pages 139-148

  10. Recombinant Genetic Libraries and Human Monoclonal Antibodies

    • Jarrett J. Adams, Bryce Nelson, Sachdev S. Sidhu
    Pages 149-170

  11. Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm

    • Lilach Vaks, Itai Benhar
    Pages 171-184

  12. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG–Single-Chain Fv Fusion

    • Dan Lu, Zhenping Zhu
    Pages 185-213

  13. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display

    • André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, Michael Hust
    Pages 215-243

  14. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice

    • Susana Magadán Mompó, África González-Fernández
    Pages 245-276

  15. Phage Display Technology for Human Monoclonal Antibodies

    • Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, Daniele Sblattero
    Pages 277-295

  16. Antigen-Specific In Vitro Immunization: A Source for Human Monoclonal Antibodies

    • Kosuke Tomimatsu, Sanetaka Shirahata
    Pages 297-307

  17. Methods for Radiolabelling of Monoclonal Antibodies

    • Vladimir Tolmachev, Anna Orlova, Karl Andersson
    Pages 309-330

  18. Purification of Human Monoclonal Antibodies and Their Fragments

    • Thomas Müller-Späth, Massimo Morbidelli
    Pages 331-351

  19. Idiotype-Specific Intravenous Immunoglobulin (IVIG) for Therapy of Autoimmune Diseases

    • Miri Blank, Tomer Bashi, Yehuda Shoenfeld
    Pages 353-361

  20. Correction to: Therapeutic Human Monoclonal Antibodies Against Cancer

    • Jamie Jarboe, Anumeha Gupta, M. Wasif Saif
    Pages C1-C1
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Keywords

About this book

The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.

Editors and Affiliations

  • Department of Pathology, The Lautenberg Center, IMRIC The Hebrew University Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

Bibliographic Information

  • Book Title: Human Monoclonal Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Steinitz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-586-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-585-9Published: 14 September 2013

  • Softcover ISBN: 978-1-4939-6019-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-586-6Published: 14 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 364

  • Number of Illustrations: 21 b/w illustrations, 17 illustrations in colour

  • Topics: Immunology, Antibodies

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