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  • © 2014

Human Monoclonal Antibodies

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1060)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases

    • Sotirios Kotsovilis, Evangelos Andreakos
    Pages 37-59
  3. Therapeutic Human Monoclonal Antibodies Against Cancer

    • Jamie Jarboe, Anumeha Gupta, M. Wasif Saif
    Pages 61-77
  4. Polyclonal and Monoclonal Antibodies in Clinic

    • Bharath Wootla, Aleksandar Denic, Moses Rodriguez
    Pages 79-110
  5. Humanization and Simultaneous Optimization of Monoclonal Antibody

    • T. Kuramochi, T. Igawa, H. Tsunoda, K. Hattori
    Pages 123-137
  6. Chimeric Antibodies

    • Kohei Kurosawa, Waka Lin, Kunihiro Ohta
    Pages 139-148
  7. Recombinant Genetic Libraries and Human Monoclonal Antibodies

    • Jarrett J. Adams, Bryce Nelson, Sachdev S. Sidhu
    Pages 149-170
  8. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display

    • André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, Michael Hust
    Pages 215-243
  9. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice

    • Susana Magadán Mompó, África González-Fernández
    Pages 245-276
  10. Phage Display Technology for Human Monoclonal Antibodies

    • Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, Daniele Sblattero
    Pages 277-295
  11. Methods for Radiolabelling of Monoclonal Antibodies

    • Vladimir Tolmachev, Anna Orlova, Karl Andersson
    Pages 309-330
  12. Purification of Human Monoclonal Antibodies and Their Fragments

    • Thomas Müller-Späth, Massimo Morbidelli
    Pages 331-351
  13. Idiotype-Specific Intravenous Immunoglobulin (IVIG) for Therapy of Autoimmune Diseases

    • Miri Blank, Tomer Bashi, Yehuda Shoenfeld
    Pages 353-361
  14. Correction to: Therapeutic Human Monoclonal Antibodies Against Cancer

    • Jamie Jarboe, Anumeha Gupta, M. Wasif Saif
    Pages C1-C1

About this book

The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.

Editors and Affiliations

  • Department of Pathology, The Lautenberg Center, IMRIC The Hebrew University Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

Bibliographic Information

  • Book Title: Human Monoclonal Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Steinitz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-586-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-585-9Published: 14 September 2013

  • Softcover ISBN: 978-1-4939-6019-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-586-6Published: 14 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 364

  • Number of Illustrations: 21 b/w illustrations, 17 illustrations in colour

  • Topics: Immunology, Antibodies

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access