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Neural Progenitor Cells

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Brent A. Reynolds

    1. Department of Neurosurgery, University of Florida, Gainesville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Loic P. Deleyrolle

    1. Department of Neurosurgery, University of Florida, Gainesville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1059)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Culturing Neural Precursor Cells from Primary Tissue

    1. Front Matter

      Pages 1-1
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    2. Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions

      • Andrew Chojnacki, Samuel Weiss
      Pages 3-12

    3. In Vitro Expansion of Fetal Neural Progenitors as Adherent Cell Lines

      • Steven M. Pollard
      Pages 13-24

    4. Isolate and Culture Precursor Cells from the Adult Periventricular Area

      • Chiara Cavazzin, Margherita Neri, Angela Gritti
      Pages 25-40

    5. Culturing and Expansion of Precursor Cells from the Adult Hippocampus

      • Dhanisha J. Jhaveri, Boris W. Prosper, Perry F. Bartlett
      Pages 41-51

    6. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

      • Jean-Philippe Hugnot
      Pages 53-63

    7. Culturing and Expansion of “Clinical Grade” Precursors Cells from the Fetal Human Central Nervous System

      • Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, Angelo Luigi Vescovi
      Pages 65-77

    8. Isolating and Culturing of Precursor Cells from the Adult Human Brain

      • Florian A. Siebzehnrubl, Dennis A. Steindler
      Pages 79-86

    9. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

      • Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat, Jean-Philippe Hugnot
      Pages 87-93

    10. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny

      • Hassan Azari
      Pages 95-106

    11. Isolation of Adult Stem Cells from the Human Olfactory Mucosa

      • François Féron, Chris Perry, Stéphane D. Girard, Alan Mackay-Sim
      Pages 107-114

  3. Stem Cells vs Progenitor Cells

    1. Front Matter

      Pages 115-115
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    2. Enumerating Stem Cell Frequency: Neural Colony Forming Cell Assay

      • Sharon A. Louis, Carmen K. H. Mak
      Pages 117-132

  4. Purification of Stem and Progenitor Cells

    1. Front Matter

      Pages 133-133
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    2. Flow Cytometry of Neural Cells

      • Geoffrey W. Osborne
      Pages 135-144

  5. Transplantation of Neural Progenitor Cells: Preclinical Models

    1. Front Matter

      Pages 145-145
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    2. Neonatal Transplant in Hypoxic Injury

      • Tong Zheng, Michael D. Weiss
      Pages 147-156

    3. Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke

      • Ricardo L. Azevedo-Pereira, Marcel M. Daadi
      Pages 157-167

    4. Transplantation of Fetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson’s Disease

      • Lachlan H. Thompson, Clare L. Parish
      Pages 169-180
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Keywords

About this book

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing “no new neuron” doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.

Editors and Affiliations

  • Department of Neurosurgery, University of Florida, Gainesville, USA

    Brent A. Reynolds, Loic P. Deleyrolle

Bibliographic Information

  • Book Title: Neural Progenitor Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Brent A. Reynolds, Loic P. Deleyrolle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-574-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-573-6Published: 11 August 2013

  • Softcover ISBN: 978-1-4939-6275-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-574-3Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 241

  • Number of Illustrations: 13 b/w illustrations, 36 illustrations in colour

  • Topics: Neurosciences, Stem Cells, Neurobiology

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