Overview
- Details methods that are used to study DNA using electrophoresis
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1054)
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Table of contents (19 protocols)
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Front Matter
Keywords
- 2-dimentional gel electrophoresis
- DNA electrophoresis
- DNA molecules
- PCR products
- Pulse Field Gel Electrophoresis
- agarose gels
- bacterial chromosomes
- dsDNA molecules
- electrophoreses
- eukaryotic chromosomes
- fluorescence in situ hybrifization
- linear DNA
- plasmid digests
- protein-DNA interactions
- ssDNA
- Electrophoresis
About this book
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Editors and Affiliations
Bibliographic Information
Book Title: DNA Electrophoresis
Book Subtitle: Methods and Protocols
Editors: Svetlana Makovets
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-565-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2013
Hardcover ISBN: 978-1-62703-564-4Published: 05 August 2013
Softcover ISBN: 978-1-4939-5998-3Published: 23 August 2016
eBook ISBN: 978-1-62703-565-1Published: 04 August 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 299
Number of Illustrations: 36 b/w illustrations, 20 illustrations in colour
Topics: Human Genetics, Electrophoresis