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Imaging and Tracking Stem Cells

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Kursad Turksen

    1. Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes key methods and protocols aimed at exploring the imaging and tracking of a wide variety of stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains expert notes and key implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1052)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny

    • Felipe Ortega, Benedikt Berninger, Marcos R. Costa
    Pages 1-11

  3. Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology

    • Marie-Therese Armentero, Patrizia Bossolasco, Lidia Cova
    Pages 13-28

  4. High-Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes

    • Gábor Földes, Maxime Mioulane
    Pages 29-39

  5. A High Content Imaging-Based Approach for Classifying Cellular Phenotypes

    • Joseph J. Kim, Sebastián L. Vega, Prabhas V. Moghe
    Pages 41-48

  6. Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions

    • Go Nagamatsu, Toshio Suda
    Pages 49-56

  7. Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells

    • Youbo Zhao, Andrew J. Bower, Benedikt W. Graf, Marni D. Boppart, Stephen A. Boppart
    Pages 57-76

  8. Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation Phenotyping of Stem Cells

    • Jian Tajbakhsh
    Pages 77-88

  9. Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention

    • HuaLei Zhang, Rong Zhou
    Pages 89-99

  10. Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice

    • Takaya Abe, Shinichi Aizawa, Toshihiko Fujimori
    Pages 101-108

  11. Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo

    • Minjung Kang, Panagiotis Xenopoulos, Silvia Muñoz-Descalzo, Xinghua Lou, Anna-Katerina Hadjantonakis
    Pages 109-123

  12. Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Postmortem Multicolor Histological Analysis

    • Kristien Reekmans, Nathalie De Vocht, Jelle Praet, Debbie Le Blon, Chloé Hoornaert, Jasmijn Daans et al.
    Pages 125-141

  13. Micro-CT Technique for Three-Dimensional Visualization of Human Stem Cells

    • Andrea Farini, Chiara Villa, Marzia Belicchi, Mirella Meregalli, Yvan Torrente
    Pages 143-152

  14. Noninvasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging

    • Annette Tennstaedt, Markus Aswendt, Joanna Adamczak, Mathias Hoehn
    Pages 153-166

  15. Magnetic Resonance Imaging and Tracking of Stem Cells

    • Hossein Nejadnik, Rostislav Castillo, Heike E. Daldrup-Link
    Pages 167-176

  16. Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intramuscular and Systemic Delivery

    • Boris Odintsov, Ju Lan Chun, Suzanne E. Berry
    Pages 177-193

  17. Molecular Imaging and Tracking Stem Cells in Neurosciences

    • Toma Spiriev, Nora Sandu, Bernhard Schaller
    Pages 195-201

  18. Bioluminescence Imaging of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction

    • Weijun Su, Liang Leng, Zhongchao Han, Zuoxiang He, Zongjin Li
    Pages 203-215

  19. Back Matter

    Pages 217-218
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Keywords

About this book

Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.  As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny.

Editors and Affiliations

  • Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Imaging and Tracking Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-559-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-558-3Published: 13 August 2013

  • Softcover ISBN: 978-1-4939-6059-0Published: 30 April 2017

  • eBook ISBN: 978-1-62703-559-0Published: 03 June 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 218

  • Number of Illustrations: 40 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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